荧光监测TB-DNA在呼吸系统疾病中的应用价值研究  

Clinical application of fluorescence monitoring of TB-DNA in respiratory diseases

作　　者：彭宁[1] 陈敏[1] 寿志南[1] Peng Ning;Chen Min;Shou Zhinan(Department of Respiratory Medicine,the First People's Hospital of Guiyang City,Guizhou Province,Guizhou Guiyang 550002)

机构地区：[1]贵州省贵阳市第一人民医院呼吸内科,贵州贵阳550002

出　　处：《中国社区医师》2021年第1期103-104,共2页Chinese Community Doctors

摘　　要：目的:探讨肺泡灌洗液与胸腔积液中TB-DNA检查在呼吸系统疾病中的应用价值。方法:选取肺部影像学异常患者113例,分别行肺泡灌洗液TB-DNA及痰找抗酸杆菌监测;再选取胸腔积液患者90例,同时行胸腔积液ADA及胸腔积液TB-DNA监测。结果:肺部影像学异常患者113例中,明确诊断肺结核27例,TB-DNA灵敏度为92.6%,痰找抗酸杆菌灵敏度为63.%,差异有统计学意义(P<0.05);非肺结核患者中无1例TB-DNA增高,特异度达93.0%。胸腔积液患者90例中,结核性胸膜炎29例,胸腔积液ADA增高5例,仅1例胸腔积液TB-DNA增高。结论:分析荧光定量PCR检测TB-DNA是临床病原菌检测的一种具有高灵敏度、高准确性、高特异度、定量范围宽、操作简单、快速安全、污染最少的可靠的DNA体外扩增技术,对临床辅助诊断肺结核具有较高的实用价值。Objective:To explore the application value of pulmonary alveolar lavage fluid and pleural effusion of TB-DNA test in respiratory diseases.Methods:113 cases of patients with pulmonary imaging abnormalities in our department were monitored by TB-DNA in alveolar lavage fluid and acid-fast bacilli in sputum.90 cases of patients with pleural effusion were simultaneously monitored by pleural effusion ADA and pleural effusion TB-DNA.Results:Among the 113 cases of patients with pulmonary imaging abnormalities,tuberculosis was definitely diagnosed in 27 cases,with a TB-DNA sensitivity of 92.6%and a sputum acid-fast bacilli sensitivity of 3.0%,the difference was statistically significant(P<0.05).Among the non-tuberculosis patients,no TB-DNA was increased,the specificity was 93.0%.Of the 90 patients with pleural effusion,29 cases had tuberculous pleurisy,5 cases had increased pleural effusion ADA,while only 1 case had increased pleural effusion TB-DNA.Conclusion:Analytical fluorescence quantitative PCR in detection of TB-DNA is a reliable DNA in vitro amplification technique with high sensitivity,high accuracy,high specificity,wide quantitative range,simple operation,rapid safety and least contamination for clinical pathogen detection,it has high practical value for clinical auxiliary diagnosis of pulmonary tuberculosis.

关 键 词：肺结核 肺泡灌洗液 胸腔积液 TB-DNA 

分 类 号：R56[医药卫生—呼吸系统]