曲面响应法优化玛咖多糖的提取条件  

Optimization of Extraction Condition of Maca Polysaccharide with Response Surface Methodology

作　　者：雍莉[1] 杨觅 罗新月 赵璇 周荣芬[3] 任琳[1] 曾红燕 邹晓莉 YONG Li;YANG Mi;LUO Xingyue;ZHAO Xuan;ZHOU Rongfen;REN Lin;ZENG Hongyan;ZOU Xiaoli(Sichuan Center for Disease Control and Prevention,Chengdu 610041,Sichuan Province,China;West China School of Public Health and West China Fourth Hospital,Sichuan University,Chengdu 610041,Sichuan Province,China;Wuhou District Disease Prevention and Control Center,Chengdu 610041,Sichuan Province,China)

机构地区：[1]四川省疾病预防控制中心,成都610041 [2]四川大学华西公共卫生学院华西第四医院,成都610041 [3]成都市武侯区疾病预防控制中心,成都610041

出　　处：《职业卫生与病伤》2020年第6期375-378,381,共5页Occupational Health and Damage

摘　　要：目的运用响应曲面法的Box-Behnken设计,研究玛咖多糖的最佳提取条件。方法苯酚-硫酸法作为多糖的测定方法。选择原料粒度、提取温度、酒精浓度作为优化因素,研究各因素的不同水平对玛咖多糖提取效率的影响。结果通过响应曲面分析得到玛咖多糖的最优提取条件为:原料粒度为0.15~0.18 mm,提取温度为10℃,酒精浓度为0%。结论所建立的回归模型有效,玛咖多糖最优理论提取效率可达13.11 mg/g,实际提取效率为12.84 mg/g,与理论值的相对误差为-2.1%。Objective Box Behnken method of Response Surface Methodology(RSM)was used to explore the optimal extraction conditions of maca polysaccharide.Methods Phenol-sulfuric acid method was used for the determination of maca polysaccharide.In RSM analysis,the effects of raw material size,extraction temperature and alcohol concentration on the extraction efficiency of maca polysaccharide were investigated.Results The optimal extraction conditions of maca polysaccharide were as follows:0.15-0.18 mm of maca granularity,10℃of extraction temperature,0%of alcohol.Conclusion The regression model established is valid,the practical extraction efficiency for maca polysaccharide was 12.84 mg/g in the experiment,which was consistent with the theoretical value(13.11 mg/g),and the relative deviation was-2.1%.

关 键 词：玛咖 多糖 提取条件优化 响应曲线法 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]