左旋肉碱对小鼠酒精性肝损伤的保护作用  

Protective effect of L-carnitine on alcoholic liver injury

作　　者：赵荣[1] 陈东亚[1] 施伟庆[1] 陈耿[1] 陆罗定[1] 陈新霞[1] ZHAO Rong;CHEN Dong-ya;SHI Wei-qing;CHEN Geng;LU Luo-ding;CHEN Xin-xia(Jiangsu Provincial Center for Disease Control and Prevention,Jiangsu Nanjing 210009,China)

机构地区：[1]江苏省疾病预防控制中心,江苏南京210029

出　　处：《江苏预防医学》2020年第6期626-628,共3页Jiangsu Journal of Preventive Medicine

摘　　要：目的探讨左旋肉碱对小鼠酒精性肝损伤的保护作用。方法将60只雄性SPF级ICR小鼠随机分成溶剂、模型对照组及3个剂量组(400、800、2400mg/kg b.wt.)。剂量组经口灌胃给予受试物,溶剂和模型对照组给予纯净水,灌胃量均为20ml/kg b.wt.,连续灌胃30d,剂量组及模型组小鼠一次灌胃14ml/kg b.wt 50%乙醇,建立急性酒精性肝损伤模型,处死动物,对各组小鼠肝组织中丙二醛(MDA)、谷胱甘肽(GSH)、甘油三酯(TG)含量进行分析和病理学检查,并对结果进行比较。结果各组小鼠灌胃前后初始、终末体重差异均无统计学意义(F值分别为0.135、0.662,P值均>0.05)。模型对照组MDA[(1.06±0.38)nmol/mg蛋白]、TG[(13.4±4.0)μmol/g肝]含量均高于溶剂对照组[分别为(0.67±0.32)nmol/mg蛋白和(8.9±4.0)μmol/g肝)],GSH含量[(7.32±2.21)mg/g蛋白)]低于溶剂对照组[(9.82±2.81)mg/g蛋白)],肝损伤模型成功建立。与模型对照组相比,中、低剂量组小鼠肝匀浆中MDA值分别为(0.79±0.19)、(0.68±0.29)nmol/mg蛋白)均降低,低剂量组GSH含量[(9.75±2.39)mg/g蛋白)]升高、TG含量[(9.3±4.4)μmol/g肝]降低,差异均有统计学意义(t值分别为-2.208、-3.101;2.632、-2.513,P值均<0.05);各剂量组小鼠肝细胞脂肪变性均观察到不同程度的减轻,低剂量组差异有统计学意义(Z=-2.226,P<0.05)。结论在试验条件下,左旋肉碱对酒精引起的肝损伤有辅助保护功能。Objective To investigate the protective effect of L-carnitine on alcoholic liver injury.Methods A total of 60 male SPF ICR mice were randomly divided into 5 groups:3 dose groups(400,800,2400 mg/kg b.wt.),solvent control group and model control group.Mice in dose groups were given with different concentrations of L-carnitine while mice in solvent control group and model control group were treated with distilled water by intragastric administration of 20 ml/kg b.wt.for 30 d administration.Mice in dose groups and model control group were given 50%ethanol(14 ml/kg b.wt.)to establish alcoholic liver injury model.After the mice were killed,the malondialdehyde(MDA),glutathione(GSH),triglyceride(TG)and histopathological levels in livers were examined and compared.Results There was no significant difference in initial body weight and final body weight of mice in every group(F=0.135 and 0.662,all P>0.05).The MDA level[(1.06±0.38)nmol/mg protein]and TG level[(13.4±4.0)μmol/g liver]in model control group were higher than those in solvent control group[(0.67±0.32)nmol/mg protein and(8.9±4.0)μmol/g liver],while GSH level[(7.32±2.21)mg/g protein]was lower than that in solvent control group[(9.82±2.81)mg/g protein];the liver damage model was established successfully.Compared with the model control group,the MDA levels in liver homogenate of medium and low dose groups[(0.79±0.19)and(0.68±0.29)nmol/mg protein]decreased,while GSH level[(9.75±2.39)mg/g protein]increased and TG level[(9.3±4.4)nmol/mg protein]decreased in low-dose group,all above differences were statistically significant(t=-0.208,-3.101;2.632,-2.513,all P<0.05).The liver steatosis of mice in each dose group reduced for different degrees,and the difference was statistically significant in the low-dose group(Z=-2.226,P<0.05).Conclusion Under experimental conditions,the L-carnitine has certain protective effect to the alcoholic liver injury in mice.

关 键 词：左旋肉碱 酒精性肝损伤 保护作用 

分 类 号：R114[医药卫生—卫生毒理学]