甘肃省孢囊线虫新记录种:艾球孢囊线虫  

Globodera artemisiae,a new cyst nematode record in Gansu,China

作　　者：韩尚明 李文豪 李惠霞 张译文 倪春辉 HAN Shangming;LI Wenhao;LI Huixia;ZHANG Yiwen;NI Chunhui(College of Plant Protection,Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃农业大学植物保护学院/甘肃省农作物病虫害生物防治工程实验室,甘肃兰州730070

出　　处：《草业科学》2020年第12期2567-2573,共7页Pratacultural Science

基　　金：国家公益性行业(农业)科研专项(201503114);甘肃省科技支撑计划(1604NKCA053-2)。

摘　　要：在2018−2019年,在甘肃省天祝县高寒草地艾蒿(Artemisia argyi)根际分离得到1个孢囊线虫群体,采用形态学结合分子生物学技术进行了种类鉴定。该孢囊线虫群体形态特征:孢囊呈褐色,球形,颈部较为突出,无阴门锥;阴门膜孔为环膜孔型,无下桥和泡状突。二龄幼虫线形,头部与体驱间缢缩不明显,口针强壮。尾部圆锥形,末端较尖锐,经形态学初步鉴定为艾球孢囊线虫(Globodera artemisiae)。对该群体ITS-rDNA和28S-rRNA D2-D3区进行扩增,分别得到948 bp和1015 bp两个片段。测序后进行聚类分析,发现该群体与艾球孢囊线虫聚为一支,置信度为97%以上。结合形态学和分子生物学特征,将该群体鉴定为艾球孢囊线虫,这也是甘肃首次报道球孢囊线虫属线虫,系甘肃省新记录种。In 2018−2019,a cyst nematode population was isolated from the rhizosphere of Artemisia argyi on an alpine grassland in Tianzhu County,Gansu Province,China.The morphological characteristics of this nematode population were:brown spherical cysts,a prominent neck,generally circumfenestrate,with no vulval cone,underbridge,or bullae.Secondstage juveniles were vermiform,with inconspicuous head constriction,and a strong stylet.The tail was conical and sharply pointed.The ITS-rDNA and 28S-rRNA D2-D3 regions of the population were amplified,from which 948 and 1015 bp fragments were obtained,respectively.After sequencing,cluster analysis showed that the population was clustered as Globodera artemisiae,with a confidence of over 97%.This population was identified as G.artemisiae based on its morphological and molecular characteristics.This is the first report of the genus Globodera and a new species record in Gansu Province.

关 键 词：艾蒿 艾球孢囊线虫 形态鉴定 分子鉴定 ITS-RDNA 28s-rRNA 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]