高原鼢鼠DNA条形码筛选  

Selection of plateau zokor(Eospalax baileyi)DNA barcodes

作　　者：王缠 潘永泰 王静[3] 刘发央[1] 姚宝辉 苏军虎[1,4,5] WANG Chan;PAN Yongtai;WANG Jing;LIU Fayang;YAO Baohui;SU Junhu(College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,Gansu,China;School of Sciences,Tibet University,Lhasa 850000,Tibet,China;Lanzhou Vocational and Technical College,Lanzhou 730070,Gansu,China;College of Grassland Science,Key Laboratory of Grassland Ecosystem(Ministry of Education),Pratacultural Engineering Laboratory of Gansu Province,Sino–U.S.Centers for Grazing land Ecosystem Sustainability,Gansu Agricultural University,Lanzhou 730070,Gansu,China;Gansu Agricultural University–Massey University Research Centre for Grassland Biodiversity,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]西藏大学理学院,西藏拉萨850000 [3]兰州职业技术学院,甘肃兰州730070 [4]甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中–美草地畜牧业可持续发展研究中心,甘肃兰州730070 [5]甘肃农业大学–新西兰梅西大学草地生物多样性研究中心,甘肃兰州730070

出　　处：《草业科学》2020年第12期2574-2583,共10页Pratacultural Science

基　　金：甘肃省教育厅科研项目(2015A-205);陇原青年创新创业人才项目(LQ20180916-2);甘肃农业大学“伏羲杰出人才”培育项目(Gaufx-02J03)。

摘　　要：高原鼢鼠(Eospalax baileyi)是青藏高原特有的啮齿类动物,具有重要的生态和经济价值。为筛选出可用于鉴定高原鼢鼠的DNA条形码,本研究采用PCR直接测序的方法测定了鼢鼠亚科高原鼢鼠4个近缘种线粒体全基因组序列,并结合GenBank中其他鼢鼠的相关序列,运用Clustal X 1.83、MEGA 5.0和Dna SP 5.0等生物信息学软件,进行了遗传距离测定和系统进化树构建等分析。结果发现,从序列的扩增成功率与已有序列信息来看,鼢鼠亚科动物线粒体基因组中的10个基因序列可供选择,其序列相似性在30.07%~98.31%;遗传距离频率分布表明Cyt b、ND4和COX1种内遗传距离小于种间遗传距离,系统进化树中检测发现基于Cyt b和ND4基因序列能将地理种群区分开来,并符合传统分类,综合分析发现Cyt b和ND4基因适合作为鉴定高原鼢鼠的DNA条形码。Plateau zokor(Eospalax baileyi)is a unique rodent from the Tibetan Plateau,which has important ecological and economic functions.DNA barcoding is emerging as a useful tool not only for species identification,but also for phylogenetic studies.To screen out DNA barcodes that could be used to identify the plateau zokor,the complete mitochondrial genome sequences of four sibling species of plateau zokor,subfamily Myospalacinae,were determined using PCR direct sequencing.The genetic distance and phylogenetic tree construction were analyzed using bioinformatics software Clustal X 1.83,MEGA 5.0,and Dna SP 5.0.There were 10 gene regions identified as potential DNA barcodes,and the sequence similarity distribution ranged from 30.07%to 98.31%.The results showed that the intraspecific genetic distance of Cyt b,ND4,and COX1 was smaller than the interspecific genetic distance.In addition,the study shows that geographic populations can be determined from Cyt b and ND4 gene sequences,which is consistent with the traditional classification method.Therefore,the comprehensive analysis of Cyt b and ND4 genes is suitable for the DNA barcoding of plateau zokor.

关 键 词：DNA条形码 基因 鼢鼠亚科 高原鼢鼠 啮齿动物 序列分析 

分 类 号：S812.6[农业科学—草业科学]