槐耳清膏对LPS致小鼠炎症反应的调节作用  

Experimental study on anti-inflammatory activity of Huaier cream in mice with LPS induced inflammation

作　　者：刘超[1] 张腾元 朱日明 高鑫芯 高慧婕[1] LIU Chao;ZHANG Tengyuan;ZHU Riming;GAO Xinxin;GAO Hui jie(College of Pharmacy,Jining Medical University,Rizhao 276826,China)

机构地区：[1]济宁医学院药学院,日照276826

出　　处：《济宁医学院学报》2020年第6期389-392,共4页Journal of Jining Medical University

基　　金：山东省自然科学基金(ZR2016HL22);山东省高等学校科技计划(J18KA297);国家大学生创新训练计划项目(201810443020)。

摘　　要：目的制备脂多糖(LPS)致炎小鼠模型,观察槐耳清膏对小鼠炎症反应的调节作用。方法ICR小鼠随机分为正常对照组、LPS组、槐耳清膏低剂量组(4mg/kg)、中剂量组(6mg/kg)和高剂量组(8mg/kg),每组10只。6d后测定小鼠脾脏指数,HE染色观察小鼠肺脏组织的病理变化;ELISA检测小鼠血清IL-6、TNF-α的表达,RT-qPCR检测小鼠脾脏IL-6、TNF-αmRNA的变化。结果与正常对照组相比,LPS组小鼠的脾脏指数(5.09±0.80)和IL-6[(52.22±8.41)pg/ml]、TNF-α[(56.40±5.97)pg/ml]、IL-6 mRNA(3.16±0.89)和TNF-αmRNA(3.02±0.84)表达水平均显著升高,小鼠肺部炎症反应增加,说明LPS致炎症模型制备成功;与LPS组相比,高剂量槐耳清膏干预后的脾脏指数(3.62±0.69)和IL-6[(37.65±6.35)pg/ml]、TNF-α[(34.94±5.12)pg/ml]、IL-6 mRNA(1.32±0.15)和TNF-αmRNA(1.29±0.19)均有显著的降低(均P<0.05)。结论槐耳清膏可抵抗由LPS所致小鼠炎症,降低炎症性细胞因子的表达,有良好的抗炎效果。Objective To observe the protective effect of Huaier cream in mouse with lipopolysaccharide(LPS)induced inflammation and to explore the mechanism of anti-inflammatory activity of Huaier cream at the level of cytokines.Methods ICR mice were randomly divided into control group,LPS group,Huaier cream high,middle and low dose(8,6 and 4 mg/kg)group.After 6 days,the spleen index were analyzed and the inflammatory changes of lung tissues in each group were observed by hematoxylin and eosin(HE)staining techniques.The expression of TNF-αand IL-6 in peripheral blood was detected by ELISA.RT-qPCR was used to detect the changes of TNF-αand IL-6 mRNA expression in mouse spleen.Results Compared with control group,LPS-treated mice showed significant increase in the spleen index(5.09±0.80),IL-6[(52.22±8.41)pg/ml],TNF-α[(56.40±5.97)pg/ml],IL-6 mRNA(3.16±0.89)and TNF-αmRNA(3.02±0.84),and inflammatory changes of lung tissues in mouse.Compared with LPS model group,the Huaier cream significantly decreased the spleen index(3.62±0.69),IL-6[(37.65±6.35)pg/ml],TNF-α[(34.94±5.12)pg/ml],IL-6 mRNA(1.32±0.15)and TNF-αmRNA(1.29±0.19),and inflammatory changes of lung tissues in mouse.Conclusion Huaier cream exhibits good anti-inflammatory effect in mouse with LPS induced inflammation.

关 键 词：槐耳清膏 LPS 炎症 IL-6 TNF-Α 

分 类 号：R285.5[医药卫生—中药学]