基于仿生纳米导线的电化学传感器对水体重金属毒性的检测分析  

Detection of Acute Toxicity of Water Using Electrochemical Sensor Based on Bionic Nanowire

作　　者：王羽 王蔚港 杨向黎 尹伶灵 商冉 李霞 陈燕 WANG Yu;WANG Wei-Gang;YANG Xiang-Li;YING Ling-Ling;SHANG Ran;LI Xia;CHEN Yan(School of Resources and Environmental Engineering,Shandong Agriculture and Engineering University,Jinan 250100,China;Shandong Provincial Veterinary Medicine Supervision Institute/Shandong Provincial Key Laboratory of Quality Safety Monitoring and Risk Assessment for Livestock and Poultry Products,Jinan 250022,China)

机构地区：[1]山东农业工程学院资源与环境工程学院,济南250100 [2]山东省兽药质量检验所山东省畜产品质量安全监测与风险评估重点实验室,济南250022

出　　处：《分析化学》2021年第1期68-75,共8页Chinese Journal of Analytical Chemistry

基　　金：山东省高等学校科技计划项目(No.J16LE56);全国大学生创新创业项目(No.201714439007);国家自然科学基金项目(No.61801274);山东省高等学校青年创新团队发展计划项目(No.2019KJF029)资助。

摘　　要：准确快速诊断水体重金属类污染物的毒性对保障饮用水安全至关重要。本研究基于大肠杆菌(E.coli)细胞膜受损的毒性响应机理,以K 3Fe(CN)6作为胞外电子受体,碳纳米管(CNTs)作为仿生纳米导线,建立了一种基于电化学传感器的水体重金属毒性检测新方法。考察了电极修饰材料、菌体浓度、电子传递体与毒性物质加入顺序、K 3Fe(CN)6浓度、菌体与毒物的接触时间等因素对传感器灵敏度的影响。在最佳实验条件下,测得单一金属离子Zn^2+、Cd^2+、Cu^2+、Ag^+和Pb^2+对E.coli毒性的半数抑制浓度(IC 50)分别为10.72、15.03、6.14、16.28和20.98 mg/L。本方法操作简单、检测成本低、检测时间短且结果准确、可靠,有望用于水体急性毒性的快速预警和筛查,具有重要的实际应用价值。It is important to rapidly diagnose heavy metal toxicity of water for drinking water safety.Based on Escherichia coli(E.coli)cell damage,in this work,a new toxicity bioassay using multi-walled carbon nanotubes(MWCNTs)as bionic nanowire and K 3Fe(CN)6 as electron transporter was developed.The influences of modified materials,optical density of E.coli,incubation time and K 3Fe(CN)6 concentration on the respiratory inhibition of E.coil were investigated.Under the best conditions,IC 50 values of single metal ions(Zn^2+,Cd^2+,Cu^2+,Ag^+ and Pb^2+)were 10.72,15.03,6.14,16.28 and 20.98 mg/L,respectively.This method had virtues of simple operation,low cost,short detection time,accurate and reliable results,and might serve as an early warning device for rapid warning and screening of water acute toxicity,thus avoiding environmental pollution and protecting public health.

关 键 词：重金属毒性 大肠杆菌 仿生纳米导线 电化学传感器 

分 类 号：O657.1[理学—分析化学] TP212.2[理学—化学]