纳米四氧化三铁基质用于基质辅助激光解吸电离质谱法分析小分子化合物  

Analysis of Small Molecule Compounds by Matrix-assisted Laser Desorption Ionization Mass Spectrometry with Fe3O4 Nanoparticles as Matrix

作　　者：赵玥祯 徐杨 龚灿 鞠钰蕊 刘兆鑫 许旭 ZHAO Yue-Zhen;XU Yang;GONG Can;JU Yu-Rui;LIU Zhao-Xin;XU Xu(School of Chemical and Environmental Engineering,Shanghai Institute of Technology,Shanghai 201418,China)

机构地区：[1]上海应用技术大学化学与环境工程学院,上海201418

出　　处：《分析化学》2021年第1期103-112,共10页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.31671928);上海市自然科学基金项目(No.15ZR1440800)资助。

摘　　要：考察了基质辅助激光解吸电离质谱(MALDI-MS)使用纳米四氧化三铁(Fe3 O4)基质分析氨基酸、寡糖和甘油三酯等小分子化合物的效果。与不同的纳米基质以及二元混合基质比较后,选择Fe3 O4纳米基质,以D,L-焦谷氨酸、D,L-天冬酸、L-脯氨酸、L-苯丙氨酸、D-(+)-蔗糖、棉子糖、三棕榈酸甘油酯和三油酸甘油酯为样品,考察了实验条件对噪音和信号强度的影响,结果表明,Fe3 O4作为MALDI基质具有在增强检测信号强度的同时降低背景噪声的特点。在正电荷检测模式下,选择激光能量为70%,采用先加基质再加分析物的点样方法进行分析,结果表明,纳米Fe3 O4基质用于检测氨基酸、寡糖和甘油三酯的重现性和灵敏度良好。在优化条件下分析L-苯丙氨酸、D-(+)-蔗糖和三油酸甘油酯,点内重复性相对标准偏差(RSD)<3.2%,点间重复性RSD<6.0%,在0.05~1.0 mg/mL范围内,测定3种样品的线性相关系数R^2>0.997,显示出良好的定量分析潜力。The effect of matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS)in analysis of small molecule compounds including amino acid,oligosaccharide and triglyceride was studied using nano triiron tetraoxide(Fe 3 O 4)matrix.By comparing with other nanoparticle matrices and organic-inorganic binary matrices in the MALDI-MS analysis of D,L-pyroglutamic acid,D,L-aspartic acid,L-proline,L-phenylalanine,sucrose,raffinose,tripalmitin and triolein,the Fe 3 O 4 nanoparticles were selected as matrix,and the effects of MALDI-MS experimental conditions on MS peak noise and signal intensity were investigated.As a result,Fe 3 O 4 presented the characteristics of increasing the MS peak strength and reducing background noise.Using nano Fe 3 O 4 matrix to cover sample,and setting MS acquisition parameters including polarity as positive and laser power of 70%,the results showed that the nano Fe 3 O 4 was an ideal matrix for the detection of amino acids,oligosaccharide and triglycerides with good reproducibility and sensitivity.The MALDI-MS analysis of D,L-pyroglutamic acid,sucrose and triolein were performed using the nano Fe 3 O 4 matrix and the in-spot repeatability RSDs were less than 3.2%and the spot-to-spot repeatability RSDs were less than 6.0%.The linear correlation coefficients(R^2)between MS peak intensity and concentrations were determined as no less than 0.997 in the concentration range of 0.05-1.00 mg/mL,which showed potential quantitative power of the MALDI-MS analysis.

关 键 词：基质辅助激光解吸电离质谱 纳米四氧化三铁 氨基酸 寡糖 甘油三酯 

分 类 号：O657.63[理学—分析化学]