胰岛素腹腔注射对2型糖尿病KKAy小鼠异常血脂调节的影响  

Effects of intraperitoneal injection of insulin on the regulation of abnormal blood glucose and blood lipid in obese type 2 diabetic KKAy mice

作　　者：夏婷 高关心 夏慧琳 孙洪范[2] 俞兰 都莉娜 孙佳慧 杨诗钰 常洁 Xia Ting;Gao Guanxin;Xia Huilin;Sun Hongfan;Yu Lan;Du Lina;Sun Jiahui;Yang Shiyu;Chang Jie(Medical Engineering Department,Inner Mongolia People's Hospital,Inner Mongolia 010017,China;Tianjin Key Laboratory of Biomedical Materials,Institute of Biomedical Engineering,Chinese Academy of Medical Sciences&Peking Union Medical College,Tianjin 300192,China;Inner Mongolia Medical University College of Computer and Information,Inner Mongolia 010110,China)

机构地区：[1]内蒙古自治区人民医院医学工程处,呼和浩特010017 [2]中国医学科学院生物医学工程研究所,天津市生物医学材料重点实验室,天津300192 [3]内蒙古医科大学计算机信息学院,呼和浩特010110

出　　处：《国际生物医学工程杂志》2020年第5期360-365,共6页International Journal of Biomedical Engineering

基　　金：2017年内蒙古自治区科技计划资助项目(201702118)。

摘　　要：目的研究胰岛素腹腔注射对2型糖尿病KKAy小鼠异常血脂调节的影响。方法采用高脂高糖饲料喂养法建立2型糖尿病小鼠模型。将建模成功的KKAy小鼠随机分为3组:腹腔注射组(n=6)、皮下注射组(n=6)和发病未治疗组(n=3)。同时选取健康C57BL/6J小鼠作为正常组(n=6),健康KKAy小鼠作为未发病组(n=6)。治疗分为两个阶段,第一阶段为期6周,腹腔注射组和皮下注射组小鼠分别进行胰岛素腹腔和皮下注射;第二阶段为期4周,腹腔注射组和皮下注射组小鼠均进行胰岛素皮下注射。其余3组均不予注射。每两周检测相关指标变化情况,包括体质量、空腹血糖、餐后2 h血糖、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)含量。结果在治疗中期改变注射方案对2型糖尿病KKAy小鼠体质量和血糖无影响的条件下,腹腔注射胰岛素对HDL-C和LDL-C的调节作用明显优于皮下注射,两种注射方案对TG的调控均有效,但对降低TC效果均不明显。结论胰岛素腹腔注射对2型糖尿病KKAy小鼠的血脂异常具有一定疗效,可促进HDL-C升高和LDL-C降低,并对降低TG具有一定作用。Objective To study the effect of insulin intraperitoneal injection on abnormal blood lipid intype 2 diabetic KKAy mice.Methods Type 2 diabetic mice model was established by feeding high fat and high sugar diet.KKAy model mice were randomly divided into intraperitoneal injection group(n=6),subcutaneous injection group(n=6)and no-treatment group(n=3).At the same time,healthy C57BL/6J mice were selected as normal group(n=6),and healthy KKAy mice as disease-free group(n=6).The treatment process was divided into two stages.The first stage consists of 6 weeks,in which the mice in the intraperitoneal and subcutaneous groups were treated with insulin intraperitoneally and subcutaneously respectively.The second stage consists of 4 weeks,in which the mice in intraperitoneal and subcutaneous groups were subcutaneously injected with insulin.The mice in the remaining 3 groups were not treated.The changes of related indicators were detected every two weeks,including body weight,fasting blood sugar,2 hours after meal blood sugar,triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C)and low density lipoprotein cholesterol(LDL-C).Results Changing the injection solution in the medium term of the treatment had no effect on the body mass and blood sugar of KKAy mice with type 2 diabetes.Under this condition,the effect of intraperitoneal injection of insulin on HDL-C and LDL-C is significantly better than that of subcutaneous injection.Besides,both injection solutions are effective in regulating TG,but the effect of reducing total cholesterol is not obvious.Conclusions The intraperitoneal injection of insulin has a certain effect on the blood lipid abnormality of type 2 diabetic KKAy mice.It can promote the increase of HDL-C,the decrease of LDL-C,and the decrease of TG.

关 键 词：注射 腹腔内 注射 皮下 胰岛素 糖尿病 2型 血脂异常 

分 类 号：R587.1[医药卫生—内分泌]