地塞米松对兔淋巴细胞辐射旁效应及其再传递拮抗作用的研究  

Study on the antagonism of dexamethasone on radiation bystander effect and its re-transmission in rabbit lymphocytes

作　　者：展浩 姚若愚 张旭光[2] Zhan Hao;Yao Ruoyu;Zhang Xuguang(Department of Radiation Therapy,Affiliated Hospital of Xuzhou Medical University,Xuzhou 221002,China;Department of Radiation Therapy,Xuzhou Cancer Hospital in Jiangsu Province,Xuzhou 221005,China)

机构地区：[1]徐州医科大学附属医院放射治疗科,江苏徐州221002 [2]江苏省徐州市肿瘤医院放射治疗科,221005

出　　处：《肿瘤研究与临床》2020年第11期776-780,共5页Cancer Research and Clinic

基　　金：江苏省自然科学基金(BK20151156)。

摘　　要：目的:观察地塞米松对兔淋巴细胞辐射旁效应及其再传递的拮抗作用。方法:用6 MV X线照射2只新西兰兔后取血浆,制备一代条件培养液;取未经照射的2只新西兰兔血浆,制备一代非条件培养液;提取5只未照射新西兰兔的淋巴细胞。用一代非条件培养液或一代条件培养液分别与从5只未照射新西兰兔提取的淋巴细胞共培养,弃培养液后换为常规培养液继续培养24 h,取培养液,即二代非条件培养液或二代条件培养液。应用各培养液单独及联合地塞米松(1 μg/ml)处理淋巴细胞,应用流式细胞术检测淋巴细胞的凋亡率。结果:与一代非条件培养液作用后的淋巴细胞相比,有、无地塞米松一代条件培养液处理的淋巴细胞凋亡率均升高,差异均有统计学意义[无地塞米松:(21.09±1.60)%比(8.06±0.65)%, t=-30.182, P<0.05;有地塞米松:(14.96±1.80)%比(6.25±0.54)%, t=-16.404, P<0.05],地塞米松均可以降低一代非条件培养液和一代条件培养液处理的淋巴细胞的凋亡率,差异均有统计学意义(均 P<0.05)。与二代非条件培养液作用后的淋巴细胞相比,有、无地塞米松二代条件培养液处理的淋巴细胞凋亡率均升高,差异均有统计学意义[无地塞米松:(28.70±2.14)%比(12.38±0.67)%, t=-33.351, P<0.05;有地塞米松:(20.21±1.96)%比(12.53±1.25)%, t=-14.145, P<0.05],地塞米松可以降低二代条件培养液处理的淋巴细胞的凋亡率( P<0.05),但未降低二代非条件培养液处理的淋巴细胞的凋亡率( P>0.05)。 结论:地塞米松能够体外拮抗淋巴细胞辐射旁效应的损伤,降低淋巴细胞的凋亡率,并可以拮抗旁效应的再传递。Objective To observe the antagonism of dexamethasone on radiation bystander effect and its re-transmission in rabbit lymphocytes.Methods The plasmas of 2 New Zealand rabbits which were irradiated with 6 MV X-ray was taken to prepare the first generation of conditioned medium;the plasmas of 2 unirradiated New Zealand rabbits were taken to prepare the first generation of unconditioned culture medium;The lymphocytes of 5 unirradiated New Zealand rabbits were extracted.The lymphocytes from 5 unirradiated New Zealand rabbits were cocultured with the first generation of unconditioned medium or conditioned medium.After abandoning the culture medium,the lymphocytes were cultured in conventional medium for 24 h.The second generation of unconditioned medium or conditioned medium was taken.The lymphocytes were treated with four medium with or without dexamethasone(1μg/ml),and the apoptosis rate of lymphocytes was detected by flow cytometry.Results With or without dexamethasone,the apoptosis rates of lymphocytes treated with the first generation of conditioned medium was significantly higher than that with the first generation of unconditioned medium[without dexamethasone:(21.09±1.60)%vs.(8.06±0.65)%,t=-30.182,P<0.05;with dexamethasone:(14.96±1.80)%vs.(6.25±0.54)%,t=-16.404,P<0.05].Dexamethasone could reduce the apoptosis rates of lymphocytes treated with the first generation of unconditioned medium and the first generation of conditioned medium,and the differences were statistically significant(both P<0.05).With or without dexamethasone,the apoptosis rates of lymphocytes treated with the second generation of conditioned medium was significantly higher than that with the second generation of unconditioned medium[without dexamethasone:(28.70±2.14)%vs.(12.38±0.67)%,t=-33.351,P<0.05;with dexamethasone:(20.21±1.96)%vs.(12.53±1.25)%,t=-14.145,P<0.05].Dexamethasone could reduce the apoptosis rates of lymphocytes treated with the second generation of conditioned medium(P<0.05),but it could not reduce the apoptosis rate of

关 键 词：淋巴细胞 辐射旁效应 再传递 地塞米松 

分 类 号：R965[医药卫生—药理学]