Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere  

作　　者：Lu Song Can Wang Yizhu Wang 

机构地区：[1]Indoor Air Environment Quality Control Laboratory of Tianjin,School of Environment Science and Engineering,Tianjin University,Tianjin 300350,China

出　　处：《Frontiers of Environmental Science & Engineering》2020年第6期41-51,共11页环境科学与工程前沿（英文）

基　　金：This study was supported by the National Natural Science Foundation of China(Grant No.51678402);the key technologies R&D program of Tianjin(No.20ZXGBSY00100).

摘　　要：Antibiotic resistance genes(ARGs)have been detected in various atmospheric environments.Airborne ARGs transmission presents the public health threat.However,it is very difficult to quantify airborne ARGs because of the limited availability of collectable airborne particulate matter and the low biological content of samples.In this study,an optimized protocol for collecting and detecting airborne ARGs was presented.Experimental results showed that recovery efficiency tended to increase initially and then declined over time,and a range of 550-780 copies/mmz of capture loading was recommended to ensure that the recovery efficiency is greater than 75%.As the cell walls were mechanically disrupted and nucleic acids were released,the buffer wash protects ARGs dissolution.Three ratios of buffer volume to membrane area in buffer wash were compared.The highest concentrations of airborne ARGs were detected with 1.4μL/mm^2 buffer wash.Furthermore,the majority of the cells were disrupted by an ultrasonication pretreatment(5 min),allowing the efficiency ARGs detection of airborne samples.While,extending the ultrasonication can disrupt cell structures and gene sequence was broken down into fragments.Therefore,this study could provide a theoretical basis for the efficient filter collection of airborne ARGs in different environments.An optimized sampling method was proposed that the buffer wash was 1.4 nL/mm and the ultrasonication duration was 5 min.The indoor airborne ARGs were examined in accordance with the improved protocol in two laboratories.The result demonstrated that airborne ARGs in an indoor laboratory atmosphere could pose the considerable health risk to inhabitants and we should pay attention to some complicated indoor air environment.

关 键 词：Airborne tetracycline resistance genes Filter sampling Capture loading Membrane pretreatment Indoor laboratory atmosphere 

分 类 号：X51[环境科学与工程—环境工程]