支气管肺发育不良小鼠肺组织3型固有淋巴细胞(ILC3)数量及相关细胞因子IL-17和IL-22分泌增加  

The number of ILC3 and their related cytokines IL-17 and IL-22 increase in lungs of mice with bronchopulmonary dysplasia

作　　者：蔡佳玉 卢红艳[1] 苏兆亮[2] 王秋霞[1] 宓兰兰 徐苏晴 薛正阳 CAI Jiayu;LU Hongyan;SU Zhaoliang;WANG Qiuxia;MI Lanlan;XU Suqing;XUE Zhengyang(Department of Pediatrics,Affiliated Hospital of Jiangsu University,Zhenjiang 212000,China;International Genome Center,Jiangsu University,Zhenjiang 212000,China)

机构地区：[1]江苏大学附属医院儿科,江苏镇江212000 [2]江苏大学国际基因组学研究中心,江苏镇江212000

出　　处：《细胞与分子免疫学杂志》2020年第10期865-870,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81741052);镇江市社会发展项目(SH2017009)。

摘　　要：目的探讨3型固有淋巴细胞(ILC3)在支气管肺发育不良(BPD)小鼠肺组织中的动态变化。方法40只新生C57BL/6小鼠随机分为空气组和高氧组,每组20只。将孕19 d提前剖出的C57BL/6小鼠暴露于850 mL/L O2中复制BPD模型。在小鼠生后1、3、7、14 d,每组各处死5只小鼠,取新鲜肺组织,HE染色观察小鼠肺组织病理改变,ELISA检测肺组织匀浆中ILC3下游细胞因子白细胞介素17(IL-17)、IL-22、粒细胞-巨噬细胞集落刺激因子(GM-CSF)蛋白含量,流式细胞术检测ILC3在肺组织淋巴细胞中所占比例,IL-17^+ILC3、IL-22^+ILC3比例。结果小鼠肺组织ILC3占淋巴细胞比例在出生后7 d达到最高。与空气组相比,同时间点高氧组小鼠肺组织ILC3比例明显增加,IL-17和IL-22含量明显增加、GM-CSF含量无明显变化。与同时间点空气组相比,高氧组IL-17^+ILC3及IL-22^+ILC3比例明显增加。结论ILC3分泌IL-17及IL-22与BPD有关。Objective To investigate dynamic changes of type 3 innate lymphoid cells(ILC3)in lungs of mice with bronchopulmonary dysplasia(BPD).Methods Forty newborn C57BL/6 mice were randomized into air group and the hyperoxia group,20 mice in each group.C57BL/6 newborn mice were delivered by caesarean section on the 19th day of pregnancy and exposed to 850 mL/L O2 for replication of the BPD model.Five mice in each group were sacrificed 1 day,3,7,14 days after they were born for procurement of fresh lung tissues.HE staining was used to observe the pathological changes of lung tissues.ELISA was used to detect the protein content of downstream cytokines interleukin-17(IL-17),IL-22 and granulocyte-macrophage colony stimulating factor(GM-CSF)in lung homogenate.Flow cytometry was used for measuring the proportion of ILC3 in lymphocytes as well as the proportions of IL-17^+ILC3 and IL-22^+ILC3 in the lung.Results The proportion of ILC3 in lung tissues reached the peak on the 7th day after birth.In contrast with the air group,the proportion of ILC3 in the hyperoxia group was significantly elevated at the same time points.The protein content of IL-17 and IL-22 in the hyperoxia group went up significantly in comparison with those in the air group at the same time points,while the GM-CSF content in the hyperoxia group showed no significant changes.The proportions of IL-17^+ILC3 and IL-22^+ILC3 in the hyperoxia group significantly increased as compared with those in the air group at the same time points.Conclusion The secretion of IL-17 and IL-22 derived from ILC3 is associated with BPD.

关 键 词：支气管肺发育不良(BPD) 3型固有淋巴细胞(ILC3) 白细胞介素17(IL-17) IL-22 粒细胞-巨噬细胞集落刺激因子(GM-CSF) 

分 类 号：R563.12[医药卫生—呼吸系统] R392.12[医药卫生—内科学]