ZmSRL5 is involved in drought tolerance by maintaining cuticular wax structure in maize  

作　　者：Zhenyuan Pan Min Liu Hailiang Zhao Zengdong Tan Kun Liang Qin Sun Dianming Gong Haijun He Wenqi Zhou Fazhan Qiu 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China [2]Crop Research Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China

出　　处：《Journal of Integrative Plant Biology》2020年第12期1895-1909,共15页植物学报（英文版）

基　　金：We are grateful for the financial support for the study bythe National Key Research and Development Programof China(2016YFDo100303);National NatureScience Foundation of China(31771796 and 31860383).

摘　　要：Cuticular wax is a natural barrier on terrestrial plant organs,which protects plants from damages caused by a variety of stresses.Here,we report the identification and functional characterization of a cuticular-wax-related gene,Zea mays L.SEMI-ROLLED LEAF 5(ZmSRL5).The loss-of-function mutant srl5,which was created by a 3,745 bp in-sertion in the first intron that led to the premature tran-script,exhibited abnormal wax crystal morphology and distribution,which,in turn,caused the pleiotropic pheno-types including increased chlorophyll leaching and water loss rate,decreased leaf temperature,sensitivity to drought,as well as semi-rolled mature leaves.However,total wax amounts showed no significant difference between wild type and semi-rolled leaf5(srl5)mutant.The phenotype of srl5 was confirmed through the generation of two allelic mutants using CRISPR/Cas9.ZmSRL5 encodes a CASPARIAN-STRIP-MEMBRANE-DOMAIN-LIKE(CASPL)protein located in plasma membrane,and highly expressed in developing leaves.Further analysis showed that the expressions of the most wax related genes were not affected or slightly al-tered in srl5.This study,thus,primarily uncovers that ZmSRL5 is required for the structure formation of the cu-ticular wax and could increase the drought tolerance by maintaining the proper cuticular wax structure in maize.

关 键 词：DROUGHT STRUCTURE CASP 

分 类 号：S513[农业科学—作物学]