消渴平合剂对糖尿病肾病db/db小鼠NF-κB、IL-6表达的影响  

Effect of Xiaokeping Mixture(消渴平合剂)on Expressions of NF-κB and IL-6 in Diabetic Kidney Disease db/db Mice

作　　者：胡爽 辛传伟[1] 余霞丽 夏仲尼[1] 羊波 吕立嵩 HU Shuang;XIN Chuanwei;YU Xiali;XIA Zhongni;YANG Bo;LV Lisong(Zhejiang Academy of Chinese Medicine,Tongde Hospital of Zhejiang Province,Hangzhou,Zhejiang 310012)

机构地区：[1]浙江省中医药研究院,浙江省立同德医院,浙江杭州310012

出　　处：《中国中医药科技》2021年第1期21-24,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：国家自然科学基金面上面目(81774270);2017年浙江省医坛新秀计划项目;同德“111”人才计划项目。

摘　　要：目的:观察消渴平合剂对糖尿病肾病db/db小鼠肾脏NF-κB(核因子-κB)、IL-6(白介素-6)表达的影响,探讨其防治糖尿病肾病的机制。方法:选取24只db/db小鼠,随机分为模型组、厄贝沙坦治疗组、消渴平合剂治疗组,每组各8只,另取8只db/m小鼠作为正常对照组;各组小鼠分别连续给药8周后,检测各组小鼠糖化血清蛋白、三酰甘油、胆固醇、低密度脂蛋白、高密度脂蛋白的变化。留取肾组织,采用荧光定量PCR检测肾脏NF-κB p65mRNA、IL-6 mRNA的表达,Western blot检测肾脏NF-κB p65蛋白、IL-6蛋白的表达。结果:消渴平合剂可以明显降低db/db小鼠糖化血清蛋白水平,可明显降低db/db小鼠胆固醇、三酰甘油以及低密度脂蛋白水平,显著升高高密度脂蛋白水平。荧光定量PCR及Western blot结果显示,与模型组相比,消渴平治疗组显著降低NF-κB p65mRNA、IL-6 mRNA的表达(P<0.05),可明显抑制NF-κB p65蛋白、IL-6蛋白的表达(P<0.05)。结论:消渴平合剂可明显降低db/db小鼠糖化血清蛋白水水平,调节血脂,其机制可能与抑制肾脏NF-κB p65、IL-6基因和蛋白的表达有关。Objective:To observe the effects of Xiaokeping Mixture on the expressions of NF-κB and IL-6 in the kidney of db/db mice,and to explore the mechanism of its prevention and treatment of diabetic kidney disease.Methods:24 db/db mice were randomly divided into model group,Xiaokeping treatment group and Irbexatan group with 8 mice in each group,another 8 db/m mice were selected as the normal control group.Changes of glycosylated serum protein,triglyceride,cholesterol,low-density lipoprotein and high-density lipoprotein in mice of each group were detected after 8 weeks of continuous administration.Kidney tissues were collected and and the expressions of NF-κB p65 and IL-6mRNA in the kidney were detected by fluorescence quantitative PCR,the expressions of NF-κB p65 and IL-6 proteins in the kidney were detected by Western blot.Results:Xiaokeping Mixture could significantly reduce the level of glycosylated serum protein in db/db mice.It could significantly reduce cholesterol,triglyceride and low-density lipoprotein levels in db/db mice,and significantly increase high-density lipoprotein levels.Quantitative PCR and Western blot results indicated that,Xiaokeping Mixture could significantly reduce the expressions of NF-κB p65 and IL-6mRNA(P<0.05),inhibit the expressions of NF-κB p65 and IL-6 proteins(P<0.05),when compared with the model group.Conclusion:Xiaokeping Mixture can significantly reduce the glycosylated serum protein and regulate blood lipids in db/db mice,and the mechanism may be related to inhibiting the expression of NF-κB p65 and IL-6 genes and proteins in the kidney.

关 键 词：消渴平合剂 糖尿病肾病 核因子-ΚB 白介素-6 DB/DB小鼠 

分 类 号：R285.5[医药卫生—中药学]