水蛭对局灶节段性肾小球硬化大鼠肾脏MMP-9、TIMP-1蛋白表达的影响  

Effects of Shuizhi(Leech)on Expressions of MMP-9 and TIMP-1 in Kidney of Focal Segmental Glomerulosclerosis Rat

作　　者：杨馨 刘娜 张佩青 王海艳 陈明 YANG Xin;LIU Na;ZHANG Peiqing;WANG Haiyan;CHEN Ming(Heilongjiang Academy of Chinese Medicine Sciences,Harbin,Heilongjiang 150036)

机构地区：[1]黑龙江省中医药科学院,黑龙江哈尔滨150036

出　　处：《中国中医药科技》2021年第1期24-27,共4页Chinese Journal of Traditional Medical Science and Technology

摘　　要：目的:观察水蛭对局灶节段性肾小球硬化(FSGS)模型大鼠肾脏基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶抑制物-1(TIMP-1)蛋白表达的影响。方法:采用单侧肾切除术后两次尾静脉注射阿霉素建立大鼠FSGS模型。实验动物分为正常组、假手术组、模型组、水蛭组4组。分别于给药后4周、8周末采用免疫组化二步法检测实验大鼠肾组织MMP-9、TIMP-1蛋白表达。结果:水蛭可以增加MMP-9蛋白表达(P<0.01),减少TIMP-1蛋白表达(P<0.01)。结论:水蛭可以通过增加MMP-9蛋白的表达,减少TIMP-1蛋白的表达,从一定程度上干预细胞外基质积聚的过程,从而延缓局灶节段性肾小球硬化的进程。Objective:To observe the effects of Leech on expressions of MMP-9 and TIMP-1 in kidneys of rats with focal segmental glomerulosclerosis(FSGS).Methods:Focal segmental glomerulosclerosis rats were established by unilateral nephrectomy,then injected with adriamycin by 4 mg/kg and 3 mg/kg respectively into tail vein.The rats were randomly divided into four groups:normal group,sham operation group,model group,Leech group,each group was given distilled water or Leech 0.9 g/(kg,d),rats were killed at 4 and 8 weeks respectively after medication.The expressions of MMP-9 and TIMP-1 in kidney tissues were detected by immunohistochemical method.Results:The expression of MMP-9 protein was increased in Leech group,that was more than that of model group(P<0.01);but the expression of TIMP-1 protein was decreased in Leech group,that was lesser than that of model group(P<0.01).Conclusion:Leech can increase the expression of MMP-9 protein while decrease the expression of TIMP-1 protein,so it can intervene in the process of extracellular matrix accumulation,and slow down the progression of focal segmental glomerulosclerosis.

关 键 词：局灶节段性肾小球硬化 阿霉素 细胞外基质 基质金属蛋白酶-9 基质金属蛋白酶抑制物-1 水蛭 

分 类 号：R285.5[医药卫生—中药学]