针刀干预兔膝骨关节炎后血清中TGF-β1和Smads蛋白的差异性表达  

Differential expression of TGF-β1 and Smads protein in serum after needle knife intervention in rabbit knee osteoarthritis

作　　者：杨郁鹏 赵季宇 李佳茹 曹韵茗 闫婧 郝重耀 金晓飞 Yang Yupeng;Zhao Jiyu;Li Jiaru;Cao Yunming;Yan Jing;Hao Chongyao;Jin Xiaofei(Shanxi University of Chinese Medicine,jinzhong shanxi 030619)

机构地区：[1]山西中医药大学,山西晋中030619

出　　处：《山西中医药大学学报》2020年第6期401-403,共3页Journal of Shanxi University of Chinese Medicine

基　　金：山西省自然科学基金面上项目(201801D121353)。

摘　　要：目的:通过针刀对兔膝骨关节炎(kneeosteoarthritis,KOA)血清中转化因子β1(TGF-β1)和Smad蛋白表达的调控,继而探讨针刀的作用机理。方法:将6月龄日本大耳白兔40只,随机等分为空白组、模型组、电针组、针刀组。除空白组外其余各组采用石膏对兔左后肢伸直位制动,造膝骨关节炎模型,后针灸组采取常规针刺隔日治疗,1周3次。针刀组行松解术,1周1次。3周后采用酶联免疫吸附试验,检测兔血清中TGF-β1和p-Smad2、p-Smad3的蛋白表达。结果:经治疗后检测,针灸组、针刀组TGF-β1含量显著上升,p-Smad2和p-Smad3含量明显下降,与模型组相比均有有统计学意义(P<0.01);且针刀组与针灸组相比较,针刀组TGF-β1的上升,磷酸化信号转导分子2(P-SMAD2)、磷酸化信号转导分子3(P-SMAD3)的下降更加显著,有统计学意义(P<0.01)。结论:针刀相比针灸在膝关节骨性关节炎中使得血清中因子和蛋白的表达改变更加显著,可能是其可能更好地作用于相关信号通路得以实现的。Objective:To regulate the expression of transforming factors TGF-β1 and Smad protein in serum of rabbit knee osteoarthritis(Kneeosteoarthritis,KOA)by needle knife,and then to explore the mechanism of needle knife.Methods:Forty 6-month-old Japanese white rabbits were randomly divided into blank,model,acupuncture,and needle knife groups.Except for the blank group,the rest of the groups used plaster to immobilize the rabbit′s left hind limbs in a straight position to create a knee osteoarthritis model.The posterior acupuncture group was treated with conventional acupuncture every other day,3 times a week.The needle-knife set performs lysis,once a week.After 3 weeks,an enzyme-linked immunosorbent assay was used to detect the protein expression of TGF-β1,Smad2,and Smad3 in rabbit serum.Results:After treatment,the content of TGF-β1 in the acupuncture group and the needle-knife group increased significantly,and the content of p-Smad2 and p-Smad3 decreased significantly.Compared with the model group,there was statistical significance(P<0.01);and the needle-knife group Compared with the acupuncture group,the increase of TGF-β1,the decrease of p-Smad2 and p-Smad3 in the needle-knife group were more significant and statistically significant(P<0.01).Conclusion:Compared with acupuncture and moxibustion,in knee osteoarthritis,the expression of factors and proteins in the serum changes more significantly,increasing the content of TGF-β1 and decreasing the content of p-Smad.Therefore,the effects of acupuncture on the disease in the experiment The therapeutic advantage is significant,which may be achieved by its possible better effect on related signal pathways.

关 键 词：膝关节骨性关节炎 信号通路 蛋白表达 针刀疗法 

分 类 号：R246[医药卫生—针灸推拿学]