源自3种茄科蔬菜土壤木霉菌的分离与鉴定  

Isolation and Identification of Trichoderma spp. from Three Solanaceae Vegetables Soils

作　　者：唐琳[1] 张焕丽 TANG Lin;ZHANG Huanli(Luoyang Normal University,Luoyang 471934;Luoyang Academy of Agriculture and Forestry Sciences,Luoyang 471000,China)

机构地区：[1]洛阳师范学院,河南洛阳471934 [2]洛阳农林科学院,河南洛阳471000

出　　处：《东北农业科学》2020年第5期52-56,共5页Journal of Northeast Agricultural Sciences

基　　金：河南省高等学校重点科研项目(17A210025);洛阳师范学院应用科学与技术研究基金(180131213033)。

摘　　要：为了明确不同茄科蔬菜土壤中木霉菌的种类,本研究从源自辣椒、茄子和番茄栽培土壤中,共分离出木霉菌97株,采用形态学和分子生物学鉴定方法对菌株进行鉴定。共鉴定出4种木霉,分别是哈茨木霉、绿色木霉、拟康宁木霉和黄绿木霉。4种木霉在3种蔬菜土壤中的分布存在差异。哈茨木霉和绿色木霉在3种蔬菜土壤中均有分布,哈茨木霉菌株的数量最多,其分离频率为46.39%。本研究结果为探明特定蔬菜土壤木霉菌的分布和生防木霉的开发提供理论依据。In order to identify the species of Trichoderma spp. in different Solanaceae vegetables soils, 97 strains of Trichoderma spp. were isolated from the soil of pepper, eggplant and tomato. Morphological and molecular identification methods were used to identify the strains. The results showed that four Trichoderma spp. species were identified, including Trichoderma harzianum, Trichoderma viride, Trichoderma koningiopsis and Trichoderma aureoviride.The distribution of strains in the three soils was different. T. harzianum and T. viride were distributed in the three vegetable soils. The number of T. harzianum strains was the most, with 46.39% of separation frequency. The results of this study provide a theoretical basis for the identification of the distribution of Trichoderma spp. in specific vegetables soils and the exploitation of biocontrol agent.

关 键 词：茄科蔬菜土壤 木霉菌 分离 鉴定 

分 类 号：S476[农业科学—农业昆虫与害虫防治] Q93[农业科学—植物保护]