何首乌-穿心莲间作对何首乌根际土壤放线菌群落结构和多样性的影响  

Effect of Polygonum multiflorum-Andrographis paniculata intercropping system on rhizosphere soil actinomycetes community structure and diversity of P. multiflorum

作　　者：刘长征 周良云 廖沛然 钟磊 蔡启忠 姜晓琳 杨全 LIU Chang-zheng;ZHOU Liang-yun;LIAO Pei-ran;ZHONG Lei;CAI Qi-zhong;JIANG Xiao-lin;YANG Quan(Guangdong Provincial Research Center on Good Agricultural Practice&Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials,Guangzhou Comprehensive Experimental Station Chinese Materia Medica Industry Technology System,Key Laboratory for Production&Development of Cantonese Medicinal Materials Under State Administration of Traditional Chinese Medicine,School of Traditional Chinese Mediciney Guangdong Pharmaceutical University,Guangzhou 510036,China)

机构地区：[1]广东药科大学中药学院国家中医药管理局岭南药材生产与开发重点研究室国家中药材产业技术体系广州综合试验站广东省南药规范化种植与综合开发工程技术研究中心,广东广州510006

出　　处：《中国中药杂志》2020年第22期5452-5458,共7页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划项目(2017YFC1700704);广东省岭南中药材保护资金专项(粤财社[2017]60号);广东省普通高校青年创新人才类项目(2018KQNCX133);广东省科技项目(2017A020213023)。

摘　　要：为探索何首乌-穿心莲间作对何首乌根际土壤放线菌的影响,该研究以原始土壤为对照,何首乌在单作和间作2种种植模式下根际土壤为处理,采用高通量测序技术研究土壤放线菌的群落结构和多样性。测序结果获得了655 221条有效序列,平均长度为408 bp,OTU覆盖率和稀释性曲线表明该测序可以代表土壤放线菌的真实情况;由Alpha多样性分析结果可知土壤放线菌多样性的大小顺序为"原始土壤>间作土壤>单作土壤";单作和间作均显著改变了放线菌的群落结构,使优势属的相对丰度发生显著变化,其中单作改变最大;OTU主成分分析和聚类分析显示,所有土壤样本被分为具有明显区别的3组,并且原始土壤与间作土壤的相似性更高;间作还增加了北里孢菌属和分枝杆菌属等有益土壤放线菌属的相对丰度,有利于保持土壤健康,减少土传病害的发生。结果表明,何首乌-穿心莲间作可以减少何首乌单作所造成的土壤放线菌群落结构改变和多样性减小的现象,使何首乌根际土壤的放线菌群落更加接近原始土壤。To investigate the effect of Polygonum multiflorum-Andrographis paniculata intercropping system on rhizosphere soil actinomycetes of P. multiflorum, the community structure and diversity of soil actinomycetes were studied by using the original soil as the control group and the rhizosphere soil actinomycetes communities of P. multiflorum under monoculture and intercropping systems as the experimental group. In this study 655 221 effective sequences were obtained with an average length of 408 bp. OTU coverage and rarefaction curve showed that the sequencing could represent the real situation of soil actinomycetes. According to the results of alpha diversity analysis, the diversity soil actinomycetes varied as follows: original soil>intercropping soil>monoculture soil. The soil actinomycetes community structure and the relative abundance of dominant genera were significantly changed by both monoculture and intercropping, especially monoculture. OTU clustering and PCA analysis of soil samples showed that all the soil samples were divided into three distinct groups and the original soil was more similar to intercropping soil. In addition, intercropping increased the relative abundance of some beneficial actinomyces, such as Kitasatospora and Mycobacterium, which was beneficial to maintain soil health and reduce the occurrence of soil-borne diseases. The results show that, P. multiflorum-A. paniculata intercropping reduced the change of community structure and the decrease of diversity of soil actinomycetes caused by P. multiflorum monoculture, and made the actinomycete community in rhizosphere soil of P. multiflorum close to the original soil.

关 键 词：间作 放线菌 何首乌 穿心莲 群落结构 多样性 

分 类 号：S154.3[农业科学—土壤学] S567.2[农业科学—农业基础科学]