白背叶中大波斯菊苷的提取分离与薄层色谱鉴别  

Extraction and separation of apigenin-7-O-glucoside and TLC identification in Folium malloti apeltae

作　　者：谢巍 杨妮 蒋受军 Xie Wei;Yang Ni;Jiang Shoujun(Department of Pharmacy,Liuzhou Maternity and Child Health Hospital of GuangXi Zhuang Autonomous Region,Affiliated Maternity Hospital and Affiliated Children’s Hospital of Guangxi University of Science and Technology,Liuzhou 545001,China;Adverse Reaction Center,Guangxi Institute for Food and Drug Administration,Nanning 530029,China)

机构地区：[1]广西壮族自治区柳州市妇幼保健院药学部,广西壮族自治区广西科技大学附属妇产医院、儿童医院,柳州545001 [2]广西壮族自治区食品药品监督管理局不良反应中心,南宁530029

出　　处：《国际中医中药杂志》2020年第11期1114-1118,共5页International Journal of Traditional Chinese Medicine

基　　金：广西自然科学基金(2010GXNSFA013233);广西科技创新能力与条件建设项目(桂科攻10124008-15);广西壮族自治区中医药管理局自筹经费科研课题(GZZC2019192、GZZC2019194)。

摘　　要：目的建立白背叶中1个黄酮成分的提取分离方法及其薄层色谱鉴别方法。方法用95%乙醇回流提取白背叶,旋蒸回收乙醇,用硅胶拌匀,以石油醚洗脱、乙酸乙酯回流提取,回收浓缩乙酸乙酯部位。取乙酸乙酯部位,上聚酰胺柱,用水和甲醇梯度洗脱,收集60%乙醇馏分,进一步分离纯化;以大波斯菊苷为对照品,白背叶为对照药材,硅胶G板为吸附剂,以三氯甲烷-甲醇-甲酸(10∶3∶0.3)为展开剂,进行薄层色谱鉴别。结果从白背叶中分离得到大波斯菊苷,建立了白背叶薄层鉴别方法,斑点清晰,分离效果好。结论该方法可准确、快速对白背叶进行鉴别,可更好地控制白背叶药材质量。Object ive To establish the extraction and separation method of flavone from the Folium malloti apeltae and TLC identification method.Methods The Folium malloti apeltae was extracted by 95%ethyl alcohol,and the ethanol recovery by rotary distillation.The extracted Folium malloti apeltae was mixed well with silicone.It was eluted with petroleum ether and extracted with ethyl acetate.Then,the concentrated ethyl acetate fraction was recovered.The ethyl acetate fraction was separated and purified on a polyamide column with gradient elution of water and methanol.The TLC identification was carried out with daposthride as the reference substance,the leaves as the reference material,the silica gel G plate as the adsorbent,and chloroform methanol formic acid(10:3:0.3)as the developing agent.Results Apigenin-7-O-glucoside obtained from Folium malloti apeltae.TLC identification method of Folium malloti apeltae was established with detectable spots and satisfactory separating result.Conclusions This method to identify Folium malloti apeltae is accurate and rapid,which can be used for the quality control of Folium malloti apeltae.

关 键 词：白背叶 大波斯菊苷 提取法 色谱法 薄层 

分 类 号：R284[医药卫生—中药学]