正交试验法优选消癌平胶囊提取工艺  

Orthogonal test method to optimize the extraction process of Xiaoaiping capsules

作　　者：邓开勇 刘福[1] 贺美波[1] 周超 Deng Kaiyong;Liu Fu;He Meibo;Zhou Chao(Department of Pharmacy,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]四川省川北医学院附属医院药剂科,南充637000

出　　处：《国际中医中药杂志》2020年第11期1119-1124,共6页International Journal of Traditional Chinese Medicine

基　　金：四川省卫生和计划生育委员会科研课题(17PJ201)。

摘　　要：目的优选消癌平胶囊的最佳水提醇沉工艺。方法采用正交试验法,以通关藤苷G含量和干浸膏率为指标,考察煎煮次数、煎煮时间、加水量对消癌平胶囊水提取工艺的影响;考察醇沉浓度、相对密度、静置时间对其醇沉工艺的影响。结果优选的水提工艺为加6倍量的水煎煮3次,每次1.5 h;优选的醇沉工艺为水提液浓缩至相对密度为1.15(60 ℃),冷却后加入乙醇,使含醇量为70%,静置12 h,除去沉淀。结论优选的水提醇沉工艺稳定可行、重现性好,可为消癌平胶囊的提取纯化提供参考。Objective To optimize water extraction and alcohol precipitation technology of Xiaoaiping capsules.Methods With the content of Tenacissoside G and dry extract yield rate as the index,to investigate the effects of extraction times,extraction time and the amount of water on extraction technology by orthogonal test;and investigate the influence of the concentration of alcohol precipitation,the relatively density of concentrated liquid and storage time on alcohol precipitation technology by orthogonal test.Results The optimal water extraction technology was 6-fold water,decocting 3 times for 1.5 h each time.The optimal alcohol precipitation technology was concentrating the water extraction to the density of 1.15(60℃),and add alcohol after cooling with 70%alcohol standing for 12 hand then removing the precipitation.Conclusions The optimized water extraction and alcohol precipitation process is stable,feasible and reproducible,which could provide reference for the extraction and purification of Xiaoaiping capsules.

关 键 词：正交试验 消癌平胶囊 水提醇沉 通关藤苷G 中药工艺改进 

分 类 号：TQ461[化学工程—制药化工]