孕前保健男性年龄与精子DNA碎片、精液常规参数的相关性分析  

The relationship between age and sperm DNA fragments,semen routine parameters in preconception care males

作　　者：庾伟坚 路瑞静 江凡[1] 陈献 梁淑馨 胡蝶 周喜友[1] YU Weijian;LU Ruijing;JIANG Fan;CHEN Xian;LIANG Shuxin;HU Die;ZHOU Xiyou(Department of Clinical Laboratory,Baoan District Maternal and Child Health Care Hospital,Shenzhen,Guangdong 518102,China)

机构地区：[1]广东省深圳市宝安区妇幼保健院检验科,广东深圳518102

出　　处：《检验医学与临床》2021年第1期30-33,37,共5页Laboratory Medicine and Clinic

基　　金：广东省深圳市宝安区科技创新计划基础研究项目(2015094、2018JD125)。

摘　　要：目的探讨孕前保健男性年龄与精子DNA碎片、精液常规参数之间的临床关联。方法收集2018年5月至2019年6月于深圳市宝安区妇幼保健院生殖健康科就诊的孕前保健检查男性1237例,依据年龄截点将其分为3组(20~29岁,30~39岁和≥40岁)。采用染色质结构分析实验检测精子DNA碎片指数(DFI)、精子成熟度,同时依据世界卫生组织第五版标准操作规程检测各项精液常规临床参数。结果≥40岁男性的前向运动精子百分率、精子存活率与20~29岁男性相比,差异均有统计学意义(P<0.05)。≥40岁男性的前向运动精子百分率、精子存活率、精液体积、不动精子百分率与30~39岁男性相比,差异均有统计学意义(P<0.05)。同时≥40岁男性精子DFI显著高于20~29岁、30~39岁的男性(P<0.001),且DFI与年龄呈正相关关系(r=0.11,P<0.0001),尤表现在≥40岁男性精子DFI与年龄呈显著性正相关关系(r=0.18,P<0.0001)。男性精子DFI与正常形态精子百分率、精液浓度、前向运动精子百分率、精子存活率、非前向运动精子百分率呈负相关关系(r=-0.21、-0.11、-0.45、-0.45、-0.21,均P<0.05);而与禁欲天数、精液体积、不动精子百分率、头部畸形精子百分率呈正相关关系(r=0.14、0.13、0.46、0.21,均P<0.05)。年龄、精液体积、正常形态精子百分率、前向运动精子百分率、精子成熟度、非前向运动精子百分率和不动精子百分率是男性精子DNA碎片的独立风险因素。结论年龄影响精子活力,精子DNA碎片显著增加;男性精子DFI与其他精液常规参数呈紧密相关,可作为一项重要的男性精子质量评价指标,为全面客观评价孕前保健男性生育能力提供了有利证据。Objective To investigate the clinical associations between the age with sperm DNA fragments index(DFI)and semen routine parameters among preconception care males.Methods A total of 1237 males undergoing the preconception care examination in the Baoan District Maternal and Child Health Care Hospital from May 2018 to June 2019 were collected and divided into the three groups(20-29 years old,30-39 years old and≥40 years old)according to the age cutoff points.The semen chromatin structure analysis(SCSA)method was utilized to detect the sperm DFI and sperm maturity,at the same time the semen routine clinical parameters were detected according to the fifth edition of the standard operating procedure by WHO.Results Compared with the 20-29 years old groups,the forward motile sperm percentage,and sperm survival rate in the males of≥40 years old manifested statistically significantly differences(P<0.001).Compared with the 30-39 years old groups,the forward motile sperm percentage,sperm survival rate,semen volume and immotile sperm percentage in the males of≥40 years old manifested statistically significantly differences(P<0.001).Meanwhile the sperm DFI in males of≥40 years old was significantly higher than that in the males of 20-29 years old and 30-39 years old(P<0.05),moreover DFI showed the positive correlation with the age(r=0.11,P<0.0001),particularly the sperm DFI and age in the males of≥40 years old demonstrated the significantly positive correlation(r=0.18,P<0.0001).The male sperm DFI was negatively correlated with and the percentage of normal morphology sperms,semen concentration,forward motile sperm percentage,sperm survival rate and non-forward motile sperm percentage(r=-0.21,-0.11,-0.45,-0.45,-0.21,P<0.05);but had the positive correlation with abstinence days,semen volume,immotile sperm percentage and abnormal head(r=0.14,0.13,0.46,0.21,P<0.05).The age,semen volume,normal morphology sperm percentage,forward motile sperm percentage,HDS,non-forward motile sperm percentage and immotile sperm percentage w

关 键 词：男性 年龄 精子DNA碎片 精液常规参数 

分 类 号：R446.19[医药卫生—诊断学]