复方黄柏液涂剂对中波紫外线致HaCaT细胞急性光损伤的修复作用  

Repair effects of Compound Cortex Phellodendri Solution Pigment on HaCaT cells acute photodamage caused by ultraviolet B

作　　者：黄艳丽 钱丽洁 陈凤娟 王雄[1] HUANG Yanli;QIAN Lijie;CHEN Fengjuan;WANG Xiong(Department of Dermatology,East Branch Hospital,Affiliated Shanghai Municipal Sixth People′s Hospital,Shanghai Jiaotong University,Shanghai 201306,China)

机构地区：[1]上海交通大学附属上海市第六人民医院东院皮肤科,上海201306

出　　处：《检验医学与临床》2021年第1期34-37,共4页Laboratory Medicine and Clinic

基　　金：上海交通大学附属第六人民医院东院院级课题(20180002)。

摘　　要：目的探讨复方黄柏液涂剂对中波紫外线(UVB)致人表皮角质形成细胞(HaCaT细胞)急性光损伤的修复作用。方法用CCK8法确定复方黄柏液最佳处理浓度。体外培养HaCaT细胞,设对照组、模型组和实验组。其中实验组加入复方黄柏液后,模型组和实验组分别予10、20、30 mJ/cm^2的UVB照射,24 h后比较各组HaCaT细胞增殖率,以及培养液中乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、过氧化物酶(CAT)、基质金属蛋白酶-1(MMP-1)表达水平。结果分别予10、20、30 mJ/cm^2的UVB照射后,发现在同等UVB剂量下,实验组HaCaT细胞增殖率、SOD水平及CAT水平显著高于模型组,LDH及MMP-1表达低于模型组,差异均有统计学意义(P<0.05)。结论复方黄柏液涂剂对UVB致HaCaT细胞急性光损伤具有防护作用。Objective To explore the repair effect of Compound Cortex Phellodendri Solution Pigment on acute light damage of human skin keratinocytes cells(HaCaT cells)caused by ultraviolet B(UVB).Methods The CCK8 method was used to determine the optimal treatment concentration of Compound Cortex Phellodendri Solution.The HaCaT cells were cultured in vitro,the control group,model group and experimental group were set,in which after adding Compound Cortex Phellodendri Solution in the experimental group,the model group and experimental groups were respectively given 10 mJ/cm^2,20 mJ/cm^2 and 30 mJ/cm^2 UVB exposure.Then the HaCaT cell proliferation rate and expression levels of lactatic dehydrogenase(LDH),superoxide dismutase(SOD),catalase(CAT)and matrix metalloproteinase-1(MMP-1)in culture solution after 24 h were compared among the various groups.Results After the respective exposure of 10 mJ/cm^2,20 mJ/cm^2 and 30 mJ/cm^2 UVB,it was found that the same dose of UVB,the HaCaT cell proliferation rate,SOD and CAT activity in the experimental group were significantly higher than those in the model group,while the LDH and MMP-1 expression levels were lower than those in the model group,and the differences were statistically significant(P<0.05).Conclusion Compound Cortex Phellodendri Solution has the protective effect on HaCaT cells aucte photodamage caused by UVB.

关 键 词：复方黄柏液涂剂 人表皮角质形成细胞 急性光损伤 

分 类 号：R758.14[医药卫生—皮肤病学与性病学]