PTEN、Ki-67在儿童非霍奇金淋巴瘤中的表达和意义  

Expression and Significance of PTEN and Ki-67 in ChildhoodNon-hodgkin Lymphoma

作　　者：黄宁波[1] 高文卫[1] 梁军 朱萌 赵仁[3] HUANG Ningbo;GAO Wenwei;LIANG Jun;ZHU Meng;ZHAO Ren(College of Basic Medicine,Ningxia Medical University,Yinchuan 750004,China;College of Pharmacology,Ningxia Medical University,Yinchuan 750004,China;The General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学基础医学院,银川750004 [2]宁夏医科大学药学院,银川750004 [3]宁夏医科大学总医院,银川750004

出　　处：《宁夏医科大学学报》2020年第12期1231-1235,共5页Journal of Ningxia Medical University

摘　　要：目的探讨第10染色体同源丢失性磷酸酶-张力蛋白酶(phosphatase and tensin homolog deleted in chromosome 10,PTEN)、Ki-67在儿童非霍奇金淋巴瘤(non-hodgkin lymphoma,NHL)中的表达及意义。方法选取2015年2月至2017年12月宁夏医科大学总医院收治的以手术切除或穿刺病理活检确诊的42例NHL患儿的石蜡包埋组织标本作为观察组,同期接受手术切除治疗的淋巴结反应性增生(lymph node reactive hyperplasia,RH)患儿42例为对照组。采用免疫组织化学(SP法)检测两组标本中PTEN、Ki-67表达情况,并分析其与NHL患儿临床病理因素的关系。结果观察组PTEN阳性表达率为20/42(47.62%),高于对照组8/42(19.05%)(P<0.05);观察组Ki-67阳性表达率26/42(61.90%),高于对照组7/42(16.67%)(P<0.05)。PTEN、Ki-67在不同血清乳酸脱氢酶(lactate dehydrogenase,LDH)水平、不同国际预后指数(international prognostic index,IPI)CNHL中的阳性表达率不同,PTEN在LDH正常者中表达率高,而Ki-67在LDH增高者中表达率高(P均<0.05);PTEN在IPI低度危险者中表达率高,而Ki-67在IPI高度危险者中表达率高(P均<0.05)。PTEN、Ki-67表达与NHL患儿性别、年龄、免疫分型、全身症状、首发部位、结外受累、临床分期均无相关性(P均>0.05)。PTEN与Ki-67在NHL组织中的表达呈负相关(r=-0.645,P<0.05),二者用于NHL早期评估一致性好(kappa值=0.4325,P=0.003)。结论PTEN、Ki-67的异常表达与儿童NHL的发生发展密切相关,联合检测二者的表达情况可能有助于判断患儿的预后。Objective To investigate the expression and significance of phosphatase and tensin homolog deleted in chromosome 10(PTEN),Ki-67 in Childhood Non Hodgkin's lymphoma(CNHL).Methods A total of 42 cases of CNHL confirmed by surgical resection or biopsy in General hospital of Ningxia Medical University from February 2015 to December 2017 were selected as observation group,and another 42 cases of lymph node reactive hyperplasia(RH)from surgical resection were selected as control group.Immunohistochemical SP method was used to detect the expression of PTEN and Ki-67 in the two groups,and their relationship with clinicopathological factors of CNHL was analyzed.Results The positive expression rate of PTEN in observation group was 20/42(47.62%),which was higher than 8/42(19.05%)in control group(P<0.05).The positive expression rate of Ki-67 in observation group was 26/42(61.90%),which was higher than 7/42(16.67%)control group(P<0.05).The positive expression rates of PTEN and Ki-67 in different serum lactate dehydrogenase(LDH)levels and different international prognostic index(IPI)CNHL were different,PTEN expression was higher in the patients with normal LDH,while Ki-67 was higher in the patients with higher LDH(P all<0.05).PTEN expression was higher in the patients with low risk of IPI,and Ki-67 was higher in the patients with high risk of IPI(P all<0.05).The expression of PETN and Ki-67 had no significant correlation with gender,age,immune classification and clinical stage of CNHL(P all>0.05).There was a negative correlation between PTEN and Ki-67 in CNHL(r=-0.645,P<0.05),the consistency of PTEN and Ki-67 in early evaluation of CNHL was good(kappa=0.435,P=0.003).Conclusion The abnormal expression of PTEN and Ki-67 is closely related to the occurrence and development of CNHL.Combined detection of the expression of PTEN and Ki-67 may help to judge the prognosis of children.

关 键 词：儿童非霍奇金淋巴瘤 第10染色体同源丢失性磷酸酶-张力蛋白酶 KI-67 免疫组织化学法 

分 类 号：R725.5[医药卫生—儿科]