亚甲基丁二酸在雄性C57BL/6J小鼠体内半数致死量和半衰期的测定  

Determination of half-life and half-lethal dose of itaconic acid in C57BL/6J male mice

作　　者：郭阳阳 雷和花 王玉兰 GUO Yang-yang;LEI He-hua;WANG Yu-lan(University of Chinese Academy of Sciences,Beijing 100049,China;State Key Laboratory of Spectroscopy and Atomic and Molecular Physics,Wuhan Institute of Physics and Mathematics,Chinese Academy of Sciences,Wuhan Magnetic Resonance Center,Wuhan 430071,China;Singapore Phenome Center,Lee Kong Chian School of Medicine,School of Biological Sciences,Nanyang Technological University,Singapore 639646,Singapore)

机构地区：[1]中国科学院大学,北京100049 [2]中国科学院精密测量科学与技术创新研究院波谱与原子分子物理国家重点实验室武汉磁共振中心,武汉430071 [3]新加坡表型中心,李光前医学院,生命科学院,南洋理工大学,新加坡639646

出　　处：《上海交通大学学报（医学版）》2020年第11期1473-1476,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家重点研发计划(2017YFC0906800)。

摘　　要：目的·测定亚甲基丁二酸(itaconic acid,ITA)在小鼠体内的半衰期和半数致死量,为评价ITA在体内的代谢速率和毒性提供直观的数据。方法·采用尾静脉注射将ITA注入小鼠体内,在不同的时间点通过眼底静脉丛采血测得ITA的浓度;同时,给药后连续观察14 d,记录受试小鼠的存活情况。结果·ITA在雄性C57BL/6J小鼠体内的半衰期为29.13 min,半数致死量为258.263 mg/kg。结论·ITA在小鼠体内的代谢速率较快,半衰期较短,属于超快速消除类药物;其在小鼠体内的半数致死量显示毒性较低。Objective·To determine the half-life and half-lethal dose of itaconic acid in mice,and provide intuitive data for the metabolic rate and toxicity of itaconic acid in mice.Methods·Itaconic acid was injected into mice through caudal vein.The concentration of itaconic acid was measured by blood collection under fundus venous plexus at different time.At the same time,the survival of the mice was recorded after injection for 14 d.Results·Under the conditions of this experiment,the half-life of itaconic acid in male C57BL/6J mice was 29.13 min,and the half-lethal dose of is 258.263 mg/kg.Conclusion·Itaconic acid has a fast metabolic rate and a short half-life in mice.Itaconic acid is less toxic,based on its half-lethal dose in mice.

关 键 词：亚甲基丁二酸 小鼠 半数致死量 半衰期 

分 类 号：R965.3[医药卫生—药理学]