基于网络药理学探究马齿苋缓解结肠炎的作用机制  

Anti-colitis Mechanism of Herba Portulacae Based on Network Pharmacology

作　　者：张子越 殷斌[2] 连锐锐 张璐[1] 刘译心 李真 孙传熙 刘月月[2] 杨世发[2] 李桂明[2] 傅剑[2] 赵增成[2] 黄中利[2] 林树乾[2] 刘远飞[1] 万仁忠[1] ZHANG Ziyue;YIN Bin;LIAN Ruirui;ZHANG Lu;LIU Yixin;LI Zhen;SUN Chuanxi;LIU Yueyue;YANG Shifa;LI Guiming;FU Jian;ZHAO Zengcheng;HUANG Zhongli;LIN Shuqian;LIU Yuanfei;WAN Renzhong(College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Tai′an 271018,China;New Veterinary Drug Research and Development Office,Poultry Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China)

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]山东省农业科学院家禽研究所新兽药研发室,济南250023

出　　处：《世界中医药》2020年第24期3748-3760,共13页World Chinese Medicine

基　　金：山东省自然科学基金项目(ZR2019MC022);家禽研究所学科团队建设项目(CXGC2018E11);山东省家禽疫病诊断与免疫重点实验室开放课题基金项目(SDPDI201809)。

摘　　要：目的:利用网络药理学探究马齿苋活性成分缓解结肠炎的作用机制。方法:基于TCMSP数据库获得其活性成分和对应靶标,利用STRING进行靶标间互作分析并筛选核心靶标,经KEGG富集分析得到马齿苋治疗疾病的相关信号通路。基于Drugbank数据库获取结肠炎的相关疾病靶标,与马齿苋成分靶标进行映射,得到马齿苋治疗结肠炎的作用机制。结果:共筛选获得马齿苋活性成分花生四烯酸、β-谷甾醇、山柰酚、木犀草素、异甜菜素、槲皮素等10个,其对应靶标共187个,核心靶标共62个,经KEGG富集分析发现核心靶标与癌症、病毒感染、炎症性肠炎和免疫调节相关通路密切相关,并通过ABCC4、ABCG2、ACAT1、ALB、ALOX5、CYP3A4、IFNG、IKKA、MPO、NOS2、NQO1、NR1I2、PPARG、PTGS1、PTGS2、TNF、XDH起到治疗结肠炎的作用。结论:马齿苋可通过抗炎、抗氧化和调节细胞增殖与凋亡发挥多靶点、多通路缓解结肠炎的作用。Objective:To explore the mechanism of the active ingredients of Herba Portulacae in relieving colitis based on network pharmacology.Methods:The active ingredients and corresponding targets were obtained based on the TCMSP database,then STRING database was used to analyze the interaction among the targets and to screen the core target,which was analyzed by KEGG enrichment to obtain the signal pathway related to Herba Portulacae.Colitis-related disease targets were obtained through the Drugbank database and the targets of the ingredients of Herba Portulacae were mapped to obtain the mechanism of treating colitis by Herba Portulacae.Results:A total of 10 active ingredients such as arachidonic acid,β-sitosterol,kaempferol,luteolin,isobetain,quercetin were obtained,with 187 corresponding targets and 62 core targets.By KEGG enrichment analysis,it was found that the core targets were closely related to cancer,viral infection,inflammatory enteritis and immune regulation-related pathways,and they played a role in the treatment of colitis through targets ABCC4,ABCG2,ACAT1,ALB,ALOX5,CYP3A4,IFNG,IKKA,MPO,NOS2,NQO1,NR1I2,PPARG,PTGS1,PTGS2,TNF and XDH.Conclusion:Herba Portulacae can play a multi-target and multi-channel role in alleviating colitis through anti-inflammatory,antioxidant,and regulating cell proliferation and apoptosis.

关 键 词：马齿苋 网络药理学 结肠炎 作用机制 

分 类 号：R282[医药卫生—中药学] R574[医药卫生—中医学]