基于纳米磁流体尸体磁共振成像血管造影的可行性研究  

Research on the feasibility of applying magnetic nanoparticle ferrofluid(MNPF) to postmortem MR angiography (PMMRA)

作　　者：夏志远 Christine Bruguier Fabrice Dedouit Silke Grabherr Marc Augsburger 刘蓓蓓 Xia Zhiyuan;Christine Bruguier;Fabrice Dedouit;Silke Grabherr;Marc Augsburger;Liu Beibei(Dian Research Center for Postmortem Imaging&Angiography,Zhejiang Dian Institute of Forensic Sciences,Hangzhou 310000,China;University Center of Legal Medicine,Lausanne-Geneva(CURML),Lausanne CH1000,Switzerland;Service de Medecine Legale,Hdpital de Rangueil,Toulouse 50032,France)

机构地区：[1]浙江迪安鉴定科学研究院迪安鉴定虚拟解剖研究中心,浙江杭州310000 [2]洛桑和日内瓦大学法医中心,瑞士洛桑CH1000 [3]兰格勒医院法医学服务中心,法国图卢兹50032

出　　处：《中国法医学杂志》2020年第6期576-581,共6页Chinese Journal of Forensic Medicine

基　　金：国家留学基金(201707070113)。

摘　　要：目的初步探究纳米磁流体(magnetic nanoparticle ferrofluid,MNPF)在尸体磁共振成像血管造影(postmortem magnetic resonance angiography,PMMRA)的可行性。方法纳米粒子γ-Fe2O3(30nm)通过油酸(oleic acid,OA)表面修饰后,均匀分散在石蜡油(paraffin oil,PO)中,制备γ-Fe2O3浓度为0~1000μg/ml的MNPF。MNPF导入试管密封后固定在MR仪扫描床上,使用T1w-SE、T2w-SE和3DT2*w-GR扫描序列获取影像数据并分析影像学特点。将γ-Fe2O3浓度为0μg/ml、25μg/ml和1000μg/ml的MNPF灌注至牛离体心脏冠脉中,使用T1wSE、T2w-SE扫描序列获取影像数据并分析影像学特点。结果当γ-Fe2O3浓度在0~100μg/ml范围时,MNPF在T1wSE影像上呈现高信号,在T2w-SE影像上呈现低信号,在T2*w-SE影像上,MNPF随着γ-Fe2O3浓度的增加信号逐渐降低直至消失。当γ-Fe2O3浓度超过100μg/ml时,将可能导致磁敏感伪影的产生,而不适合造影剂的使用。结论一定浓度范围的γ-Fe2O3脂溶性MNPF,有可能成为PMMRA的T1阳性造影剂和信号可控T2*对比剂。Objective To explore the feasibility of applying magnetic nanoparticle ferrofluid(MNPF) to postmortem magnetic resonance angiography(PMMRA). Methods MNPF of concentrations ranging from 0 to 1000μg/ml was prepared by modifying nanoparticle γ-Fe2 O3(30 nm) with oleic acid(OA) and then dispersing it in paraffin oil(PO). MNPF was transferred into tubes which were then sealed and properly fixed on the MR table. To acquire image data, a serial of MR sequences were employed, including T1 w-SE, T2 w-SE and 3 DT2*w-GR and characteristics of these images were analyzed. MNPF of γ-Fe2 O3 0μg/ml, 25μg/ml and 1000μg/ml was injected into ex situ bovine hearts, T1 w-SE and T2 w-SE sequences were used and characteristics of the acquired images were analyzed. Results When the concentration of γ-Fe2 O3 lies between 0μg/ml to 100μg/ml, MNPF has hyperintensities on T1 w images and hypointensities on T2 w images. On T2*w images, the signals of MNPF tend to decrease and then disappear when the concentration of γ-Fe2 O3 increases from 0μg/ml to 100μg/ml. When the concentration surpasses 100μg/ml, it may lead to the production of magnetic sensitive artifacts, which leads to improper application of MNPF to PMMRA. Conclusion Oily MNPF with a certain concentration range of γ-Fe2 O3 has the potential to be used as T1-positive and T2*-signal-adjustable contrast agent.

关 键 词：尸体断层影像学 尸体磁共振成像血管造影 纳米磁流体 Γ-FE2O3 30nm 

分 类 号：DF795.1[医药卫生—法医学]