Enhanced lysosome escape mediated by 1,2-dicarboxylic-cyclohexene anhydride-modified poly-L-lysine dendrimer as a gene delivery system  

作　　者：Jianmin Shen Jing Chen Jingbo Ma Linlan Fan Xiaoli Zhang Ting Yue Yaping Yan Yuhang Zhang 

机构地区：[1]School of Life Sciences,Lanzhou University,Lanzhou 730000,China [2]Shenzhen Following Precision Medical Research Institute,Shenzhen 518001,China [3]School of Basic Medical Sciences,Lanzhou University,Lanzhou 730000,China

出　　处：《Asian Journal of Pharmaceutical Sciences》2020年第6期759-776,共18页亚洲药物制剂科学（英文）

基　　金：supported by the National Natural Science Foundation of China Fund(No 81541060);Science and Technology Projects from the Science Technology and Innovation Committee of Shenzhen Municipality(grant no.JCJY20170818110340383 and JCJY20170307163529489)。

摘　　要：Antisense oligodeoxynucleotide(ASODN)can directly interfere a series of biological events of the target RNA derived from tumor cells through Watson-Crick base pairing,in turn,plays antitumor therapeutic roles.In the study,a novel HIF-1αASODN-loaded nanocomposite was formulated to efficiently deliver gene to the target RNA.The physicochemical properties of nanocomposite were characterized using TEM,FTIR,DLS and zeta potentials.The mean diameter of resulting GEL-DGL-FA-ASODN-DCA nanocomposite was about 170–192 nm,and according to the agarose gel retardation assay,the loading amount of ASODN accounted for 166.7 mg/g.The results of cellular uptake showed that the nanocomposite could specifically target to HepG2 and Hela cells.The cytotoxicity assay demonstrated that the toxicity of vectors was greatly reduced by using DCA to reversibly block the cationic DGL.The subcellular distribution images clearly displayed the lysosomal escape ability of the DCA-modified nanocomposite.In vitro exploration of molecular mechanism indicated that the nanocomposite could inhibit m RNA expression and HIF-1αprotein translation at different levels.In vivo optical images and quantitative assay testified that the formulation accumulated preferentially in the tumor tissue.In vivo antitumor efficacy research confirmed that this nanocomposite had significant antitumor activity and the tumor inhibitory rate was 77.99%.These results manifested that the GEL-DGL-FA-ASODNDCA nanocomposite was promising in gene therapeutics for antitumor by interacting directly with target RNA.

关 键 词：Antisense oligodeoxynucleotide(ASODN) Gene delivery Dendrigraft poly-L-lysines(DGL) Lysosomal escape Tumor targeting 

分 类 号：R943[医药卫生—药剂学]