InDel在种间进化关系研究中的解析力初探  

The Resolution Research of InDel Mutation in Phylogenetic Tree

作　　者：刘宇[1,2] 李春娟[1] 闫彩霞[1] 赵小波[1] 苑翠玲[1] 孙全喜 王娟[1] 单世华[1,2] LIU Yu;LI Chun-juan;YAN Cai-xia;ZHAO Xiao-bo;YUAN Cui-ling;SUN Quan-xi;WANG Juan;SHAN Shi-hua(Shandong Peanut Research Institute,Qingdao 266100,China;College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China)

机构地区：[1]山东省花生研究所,山东青岛266100 [2]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《花生学报》2020年第4期1-6,13,共7页Journal of Peanut Science

基　　金：泰山学者特聘专家项目(ts201712080);青岛市科技惠民计划项目(17-3-3-49-nsh);山东省农业科学院农业科技创新工程项目(CXGC2018E21)。

摘　　要：系统发育分析是揭示物种间进化关系的重要方法。由于插入/缺失(Insertion/Deletion,InDels)变异情况的复杂性,大多数研究进行系统发育分析时往往只考虑单核苷酸碱基替换(Single nucleotide polymorphism,SNPs),忽略了InDels的遗传变异信息。为揭示InDels在系统发育分析中的解析力,本研究对花生属13个物种叶绿体基因组全序列的遗传变异信息(SNPs和InDels)进行统计和处理,首先依据全部SNPs变异所构建系统树的拓扑结构,确定的NJ树分支系统关系揭示所需SNPs饱和变异数目。结果显示,当SNP数目达到3000个之后,拓扑结构持续保持稳定。研究发现InDels能够增加系统发育树中某些节点的分辨率,但其解析力低于同等数目的SNP的解析力。综上,InDels作为主要遗传变异形式之一,能够为准确解析系统进化关系提供有力支持。Phylogenetic analysis plays an important role in evolutionary study of within and among species.However,most studies usually consider nucleotide substitutions and ignore the InDels information due to variation complexity in the phylogenetic analysis.In order to reveal the resolution power of InDels in phylogenetic analysis,the genetic variations of 13 entire chloroplast genome sequences of genus Arachis were processed.The NJ tree was constructed to confirm the saturation number of SNPs variation for stable topological branch.The result showed that the topology would not change when the total SNPs was up to 3000.Furthermore,the results found that InDels could increase the support rate of some nodes in the phylogenetic tree,but its resolution power was lower than that of an equivalent number of SNPs.In summary,InDels,one of the mainly genetic variation forms,containing the important phylogenetic information,which can provide a good reference for more accurate phylogenetic inference.

关 键 词：系统发育分析 遗传变异 InDels信息 种间进化 

分 类 号：Q754[生物学—分子生物学] Q349.5