橡胶树橡胶生物合成调控相关基因的表达相关性分析  

Correlation analysis on expression of genes related to rubber biosynthesis regulation of rubber tree

作　　者：杨署光[1] 杨秀光 赵悦 史敏晶[1] 李言[1] 邓小敏[1] 晁金泉[1] 田维敏[1] YANG Shuguang;YANG Xiuguang;ZHAO Yue;SHI Minjing;LI Yan;DENG Xiaomin;CHAO Jinquan;TIAN Weimin(Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Rubber Biology and Genetic Resources of Rubber Tree,Minstry of Agriculture and Rural Affairs,P.R.China/State Key Laboratory Incubation Base for Hainan Provincial Cultivation&Physiology of Tropical Crops,Danzhou 571737,Hainan,China;Comprehensive Agricultural Service Center of Mengjiao Nationality Township,Cangyuan WA Autonomous County,Lincang 677401,Yunnan,China;College of Agronomy and Biotechnology,China Agricultural University,Beijing 10093,China)

机构地区：[1]中国热带农业科学院橡胶研究所、农业农村部橡胶树生物学与遗传资源利用重点实验室、省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南儋州571737 [2]云南省临沧市沧源佤族自治县勐角民族乡农业综合服务中心,云南临沧677401 [3]中国农业大学农学院,北京100193

出　　处：《广西植物》2020年第12期1790-1799,共10页Guihaia

基　　金：国家重点研发计划项目(2018YFD1000502);国家青年科学基金(31800578,31700601,31800577);国家天然橡胶产业技术体系分子育种岗位专项项目(CARS-34-GW1)。

摘　　要：该文通过q PCR获得了正常割胶条件下,9个茉莉酸信号途径关键环节基因Hb COI1、Hb JAZ1、Hb JAZ2、Hb JAZ3、Hb MYC1、Hb MYC2、Hb MYC3、Hb MYC4、Hb MYC5和6个橡胶生物合成相关基因Hb HRT2、Hb SRPP、HbREF、Hb HMGR1、Hb HRT1、Hb GAPDH在5个橡胶树魏克汉种质和5个1981’IRRDB种质胶乳中的表达数据;通过皮尔逊相关系数分析了这15个基因彼此间的表达相关性,分别获得105对基因的双变量相关系数(r12)和偏相关系数(r12.3),所有105对基因的双变量相关系数(︱r12︱)和偏相关系数(︱r12.3︱)的平均值分别为0.486±0.220和0.304±0.211,达到0.05的差异显著水平。其中,r12与r12.3方向相同的63对(60%),方向相反的42对(40%);︱r12︱<︱r12.3︱的23对(21.905%),︱r12︱>︱r12.3︱的82对(78.095%);双变量相关显著性P<0.05的76对(72.38%),P<0.01的59对(56.19%);偏相关显著性P<0.05的21对(20%),P<0.01的16对(15.24%)。结果显示,这两条途径中的基因表达彼此相关,这为基因表达谱分析中普遍采用的前提假设"功能相关基因的表达相关"提供了进一步的实验证据,可用于挖掘、筛选和预测与橡胶生物合成相关的未知基因,为研究橡胶树产量形成的分子调控机理提供理论基础。Tapping-enhanced rubber biosynthesis is closely related to the activation of jasmonic acid signaling in laticifer cells of rubber tree.The expression level of genes related to jasmonic acid signaling pathway and rubber biosynthesis were both positively correlated with the dry rubber yield.However,the exact relationship between the expression of genes involved in jasmonic acid signaling and rubber biosynthesis is not to be elucidated yet.In the present study,q PCR was used to analyze the expression of nine jasmonic acid signaling genes,Hb COI1,Hb JAZ1,Hb JAZ2,Hb JAZ3,Hb MYC1,Hb MYC2,Hb MYC3,Hb MYC4,Hb MYC5 and six rubber biosynthesis genes,Hb HRT2,Hb SRPP,HbREF,Hb HMGR1,Hb HRT1,Hb GAPDH,in laticifer cells of five Wichham germplasms and five 1981’IRRDB germplasms following tapping them with S/2 D d3 tapping system,Hb18 S was used as internal reference gene.The correlation between these 15 genes was analyzed through Pearson correlation coefficient.The bivariate correlation coefficient(r12)and partial correlation coefficient(r12.3)of 105 gene pairs were obtained with the mean±standard deviation of 0.486±0.220 and 0.304±0.211,respectively.Among them,63 gene pairs(60%)with r12 in the same direction as r12.3 and 42 genes pairs(40%)in the opposite direction;In the degree of correlation,23 gene pairs(21.905%)with|r12|less than|r12.3|,and 82 gene pairs(78.095%)with|r12|greater than|r12.3|.There 76 gene pairs(72.38%)bivariate correlation coefficient(r12)were significant correlation at P<0.05 level and 59 gene pairs(56.19%)at P<0.01 level.In contrast,there less partial correlation coefficient(r12.3)were significant correlation at P<0.05 level(21 pairs,20%)and P<0.01 level(16 pairs,15.24%).These results suggested that the expression of genes involved in these two pathways were related to each other,which provides a further evidence for the assumption that"expression correlation of functional related genes",which is widely adopted in gene expression profile analysis.It can be used for excavating,screening and predicting u

关 键 词：巴西橡胶树 割胶 茉莉酸信号途径 橡胶生物合成 基因表达 Pearson相关 

分 类 号：Q943.2[生物学—植物学]