引起生姜块茎腐烂病原菌的分离与鉴定  

Isolation and Identification of Pathogens Causing Ginger Tuber Rot

作　　者：张莞苓 薛雅蓉[1] 郭雨 左广胜 刘常宏[1] Zhang Wanling;Xue Yarong;Guo Yu;Zuo Guangsheng;Liu Changhong(College of Life Sciences,Nanjing University,Nanjing 210023,China;Beijing WLong Technology Co.,Ltd.,Beijing 100071,China)

机构地区：[1]南京大学生命科学学院,江苏南京210023 [2]北京中龙创科技有限公司,北京100071

出　　处：《山东农业科学》2020年第12期79-85,共7页Shandong Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFD0800705,2017YFC0506005);国家自然科学基金项目(31471810)。

摘　　要：生姜块茎腐烂严重影响生姜的产量和质量,分离并鉴定引起生姜块茎腐烂病原菌是控制该病的基础。本研究采用传统微生物分离与基于基因序列分析的鉴定方法,从山东省潍坊市生姜腐烂块茎中分离获得10株细菌、3株真菌。通过姜块接种法和盆栽致病性试验,发现短小芽孢杆菌E4菌株和铜绿假单胞杆菌E17菌株以及尖孢镰刀菌F01菌株均可导致生姜块茎腐烂,其中铜绿假单胞杆菌是一种新的引起生姜块茎腐烂的病原菌。Ginger tuber rot seriously affects the yield and quality of ginger in China.Isolation and identification of the pathogens causing the rot of ginger tubers were the basis of controlling the disease.In this study,10 strains of bacteria and 3 strains of fungi were obtained from the rotted ginger tubers from Weifang,Shandong Province by using the traditional microbial isolation and identification method based on gene sequence analysis.Ginger tuber inoculation and potted pathogenic test showed that Bacillus pumilus E4,Pseudomonas aeruginosa E17 and Fusarium oxysporum F01 could cause ginger tuber rot,among which,P.aeruginosa E17 was a novel pathogen causing ginger tuber rot.

关 键 词：生姜 块茎腐烂 病原菌 铜绿假单胞杆菌 

分 类 号：S432:S632.5[农业科学—植物病理学]