一个抗咪唑乙烟酸长芒苋种群的发现  

A New Imazethapyr-Resistant Population of Amaranthus palmeri

作　　者：吉美静 黄兆峰[1] 崔海兰[1] 陈景超[1] 于海燕 张金鹏 李香菊[1] JI Mei-jing;HUANG Zhao-feng;CUI Hai-lan;CHEN Jing-chao;YU Hai-yan;ZHANG Jin-peng;LI Xiang-ju(Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]中国农业科学院植物保护研究所,北京100193

出　　处：《杂草学报》2020年第3期23-27,共5页Journal of Weed Science

基　　金：国家现代农业产业技术体系建设专项(编号:CARS-25)。

摘　　要：以咪唑乙烟酸为靶标除草剂,就几种苋属杂草对咪唑乙烟酸的抗药性进行研究。结果表明,供试皱果苋、反枝苋和其中的一个长芒苋P1种群(北京种群)对咪唑乙烟酸比较敏感,而在长芒苋中发现一个对咪唑乙烟酸有抗药性的P2种群(山东种群),在4倍推荐剂量下仍能全部存活。分子检测发现该种群ALS基因的第574位和第653位氨基酸发生了单碱基突变,分别为色氨酸(Trp)突变为亮氨酸(Leu)、丝氨酸(Ser)突变为天冬氨酸(Asp),种群中上述2种突变类型所占比例分别为38.9%、58.3%,2.8%的植株未检测到突变。The resistance of several Amaranthus weeds to imazethapyr used as the target herbicide was studied.The result showed that the populations of A.retroflexus and A.viridis and P1 population of A.palmeri(Beijing population)were sensitive to imazethapyr,while a new imazethapyr-resistant P2 population of A.palmeri(Shandong population)was dicoveried.And this P2 population were still survived at 4 times recommended dose of imazethapyr.The molecular detection result showed that there were single nucleotide changes in Trp574 and Ser653 of ALS gene in this population(Trp-Leu and Ser-Asp),respectively.The proportion of two mutations accounted for 38.9%and 58.3%in the P2 population,while no mutation was found in 2.8%of the detected gene sequence.

关 键 词：苋属杂草 长芒苋 咪唑乙烟酸 抗性种群 ALS基因突变 

分 类 号：S451.2[农业科学—植物保护]