促生细菌Bacillus sp. QN2MO-1 的鉴定及对番茄生长的影响  

Identification of Probiotic Bacterium Bacillus sp.QN2MO-1 and Its Effect on Tomato Growth

作　　者：张妙宜 高祝芬[2] 唐文[2] 赵炎坤[2] 李凯[2] 王尉 谢江辉[2] 张锡炎[2] 黄绵佳[1] ZHANG Miaoyi;GAO Zhufen;TANG Wen;ZHAO Yankun;LI Kai;WANG Wei;XIE Jianghui;ZHANG Xiyan;HUANG Mianjia(College of Horticulture,Hainan University,Haikou,Hainan 570228,China;Institute of Tropical Biosciences and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China)

机构地区：[1]海南大学园艺学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《热带作物学报》2020年第12期2474-2481,共8页Chinese Journal of Tropical Crops

基　　金：国家重点研发计划项目(No.2017YFD0202105);现代农业产业技术体系建设专项资金项目(No.CARS 31)。

摘　　要：为分离和鉴定具有促生作用的内生菌,开发潜在功能的微生物菌株,本研究以诺尼(Morinda citrifolia L.)为研究对象,从植株不同组织中共分离得到621株内生细菌,经复筛得到具有促生和解磷作用的菌株,命名为QN2MO-1。基于菌株的生理生化分析和16S rDNA的序列比对,该内生细菌与Bacillus siamensis KCTC 13613T(AJVF01000043)具有99.04%的同源性。药敏性分析显示,该菌对青霉素、硫酸链霉素和氨苄西林具有明显的抗性。QN2MO-1可促进番茄种子的萌发;与对照相比,5%菌体发酵液处理能显著提高植株的生长。To identify endophytic bacteria with growth-promoting function and develop potential functional microbial strains,we isolated 621 strains from different tissues of Noni(Morinda citrifolia L.).After screening,a strain with growth-promoting and phosphate-solubilizing function was obtained and named QN2MO-1.Based on the physiological and biochemical analysis of the strain and the sequence alignment of 16S rDNA,the endophyte had 99.04%homology with Bacillus siamensis KCTC 13613T(AJVF01000043).Antibiotic sensitivity analysis showed that the strain had obvious resistance to penicillin,streptomycin sulfate and ampicillin.Compared with the control,QN2MO-1 could promote the germination of tomato seeds and 5%of fermentation broth treatment could significantly improve plant growth.

关 键 词：诺尼 暹罗芽胞杆菌 促生 种子萌发 田间试验 

分 类 号：S641.2[农业科学—蔬菜学]