美国引进甘蔗材料对梢腐病菌(Fusarium sacchari)的室内抗性评价  

Resistance Evaluation in vitro of Sugarcane Germplasms Introduced from America Against Fusarium sacchari,Causing Pokkah Boeng

作　　者：古幸灵 李毅杰 周主贵 刘红坚 梁强 段维兴 雷敬超 黄诚华 谭宏伟 刘丽敏 刘平武[1] 林善海[1,2] GU Xingling;LI Yijie;ZHOU Zhugui;LIU Hongjian;LIANG Qiang;DUAN Weixing;LEI Jingchao;HUANG Chenghua;TAN Hongwei;LIU Limin;LIU Pingwu;LIN Shanhai(College of Agriculture,Guangxi University,Nanning,Guangxi 530004,China;Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences/Sugarcane Research Center,Chinese Academy of Agricultural Sciences/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi),Ministry of Agriculture and Rural Affairs,Nanning,Guangxi 530007,China;Institute of Agro-Products Processing Science and Technology,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China)

机构地区：[1]广西大学农学院,广西南宁530004 [2]广西壮族自治区农业科学院甘蔗研究所/中国农业科学院甘蔗研究中心/农业农村部广西甘蔗生物技术与遗传改良重点实验室,广西南宁530007 [3]广西壮族自治区农业科学院农产品加工研究所,广西南宁530007

出　　处：《热带作物学报》2020年第12期2492-2500,共9页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.32060592);广西创新驱动发展专项资金项目(桂科AA17202042-3);广西自然科学基金项目(No.2016GXNSFAA380019)。

摘　　要：为了筛选抗甘蔗梢腐病的优异种质材料,采用室内离体接种方法对70份甘蔗种质材料进行梢腐病抗性鉴定。根据发病程度划分的抗性等级和聚类分析的方法综合评价种质材料的抗性。结果表明,甘蔗分蘖期对梢腐病菌的免疫力较伸长期差。参试的70个种质材料中,高抗材料6个,占8.57%;抗病材料15个,占21.43%;中抗材料13个,占18.57%;中感材料12个,占17.14%;感病材料13个,占18.57%;高感材料11个,占15.71%。在2次不同生育期接种过程中‘HoCP02-263’均未发病,很可能是对Fusarium sacchari免疫的材料。系统聚类分析的结果与材料的抗性表现基本一致。甘蔗梢腐病的抗性评价较佳时期为分蘖期。In order to screen excellent sugarcane germplasms against pokkah boeng,inoculation in vitro was used for resistance assessment of 70 sugarcane germplasms to pokkah boeng.Pokkah boeng disease resistance of the tested germplasms was comprehensively evaluated based on resistance grading and cluster analysis methods.The results showed that sugarcane at tillering stage was more susceptible to pokkah boeng than at elongation stage.Among the 70 germplasms tested,6 germplasms were of high resistance,accounted for 8.57%;15 germplasms were of resistance,accounted for 21.43%;13 germplasms were of middle resistance,accounted for 18.57%;12 germplasms were of middle sensitivity,accounted for 17.14%;13 germplasms were of sensitivity,accounted for 18.57%;11 germplasms were of high sensitivity,accounted for 15.17%.Germplasm HoCP02-263 did not infected in two inoculation,which was probably immune to Fusarium sacchari.Clustering analysis confirmed the resistance performance of the sugarcane germplasms tested in vitro.Tillering stage should be chosen for resistance evaluation to sugarcane pokkah boeng disease.

关 键 词：甘蔗 梢腐病 甘蔗镰孢菌 抗性评价 聚类分析 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]