UCP-2基因启动子区-866G/A多态性与中高海拔地区2型糖尿病关系的研究  

Association of-866G/A polymorphism in uncoupling protein 2 gene with type 2 diabetes mellitus in medium and high altitude region

作　　者：罗玮[1] 姚勇利[1] 达哇卓玛 范培云[1] 包艳秋[1] 王贝贝[1] LUO Wei;YAO Yongli;Dawazhuoma;WANG Beibei(Department of Endocrinology,Qinghai Provincial People’s Hospital,Xining 810007,China)

机构地区：[1]青海省人民医院内分泌科,西宁810007

出　　处：《中国糖尿病杂志》2020年第12期887-891,共5页Chinese Journal of Diabetes

摘　　要：目的探讨中高海拔地区T2DM患者解偶联蛋白2(UCP-2)基因启动子区-866G/A多态性,筛选出本地区T2DM患者UCP-2基因启动子区-866G/A相关危险基因,探讨其与T2DM的发病机制。方法收集出生并世居青海且无亲缘关系T2DM患者100例(T2DM组),体检健康人群100名(NC组),通过多聚酶链反应-限制性片断长度多态性分析两组UCP-2基因启动子区-866G/A基因型及等位基因频率与T2DM相关指标的关系。结果两组A/A、A/G、G/G基因型频率分布及等位基因A、G频率分布差异有统计学意义(χ2=7.829、8.252,P<0.05)。A/G、A/A基因型较G/G增加T2DM发病风险,其中A/A基因型发病风险最大(OR 2.842,95%CI 1.334~6.054);等位基因A较G增加T2DM发病风险(OR 1.743,95%CI 1.192~2.549)。T2DM组A/A、A/G、G/G基因型HbA1c依次降低,HOMA-β依次升高(P<0.05),A/A与G/G基因型HbA1c、HOMA-β差异显著(P<0.05)。多元回归分析结果显示,HbA1c、TG及UCP-2-866G/A基因多态性是T2DM发病的影响因素。结论 UCP-2基因启动子区-866G/A多态性与中高海拔地区T2DM发病相关;中高海拔地区基因型A/A携带者T2DM发生风险较高。Objective To investigate the-866 G/A polymorphism of the uncoupling protein 2(UCP-2)gene promoter in patients with type 2 diabetes mellitus(T2DM)living in middle and high altitudes region,and to find the risk allele of-866 G/A as well as its pathogenic mechanism in the above population.Methods A total of 100 unrelated T2DM patients born and lived in Qinghai were enrolled as(T2DM)group,and 100 healthy people living in Qinghai were selected as normal controls(NC)group.PCR-RFLP method was used to detect the 866 G/A genotype and allele frequency in UCP-2 gene promoter and T2DM related indicators were analyzed.Results There were significant differences in the frequency distribution of A/A,A/G,G/G genotypes and alleles A and G between the two groups(χ2=7.829,8.252,P<0.05).The A/G and A/A genotypes increased the risk of T2DM compared with G/G,in which A/A genotype had the highest risk(OR 2.842,95%CI 1.334~6.054).Allele A increased the risk of T2DM compared with allele G(OR 1.743,95%CI 1.192~2.549).In T2 DM group,HbA1c decreased,while HOMA-β increased from A/A,A/G,and G/G genotypes(P<0.05).There were significant differences in HbA1c and HOMA-β between A/A and G/G genotypes(P<0.05).Multiple regression analysis showed that HbA1c,TG and UCP-2-866 G/A gene polymorphisms were the risk factors for T2DM.Conclusion The-866 G/A polymorphism inUCP-2 gene promoter region was associated with the occurrence of T2DM in population living in middle and high-altitude region.The genotype A/A carriers had a higher risk of developing T2DM in middle and highaltitude region.

关 键 词：糖尿病 2型 中高海拔 解偶联蛋白2 

分 类 号：R587.1[医药卫生—内分泌]