茉莉酸甲酯对菊花抗蚜性的影响  

Effect of methyl jasmonate on aphid resistance of chrysanthemum

作　　者：樊婕 张雪莹 孙宪芝[1,2] 许冰雁 FAN Jie;ZHANG Xue-ying;SUN Xian-zhi;XU Bing-yan(College of Horticultural Science and Engineering,Shandong Agricultural University,Tai'an 271018,China;Shandong China-Japan-Korea Chrysanthemum Cooperative Research Center,Tai'an 271018,China)

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018 [2]山东省中日韩菊花合作研究中心,山东泰安271018

出　　处：《应用生态学报》2020年第12期4197-4205,共9页Chinese Journal of Applied Ecology

基　　金：国家重点研发计划项目(2019YFD1001505)资助。

摘　　要：本研究以杭白菊为试验材料,分析茉莉酸甲酯对菊花抗蚜性的影响。供试幼苗叶面喷施不同浓度(0.01、0.05、0.1、0.5、1 mmol·L-1)茉莉酸甲酯后接种菊姬长管蚜,测定外源茉莉酸甲酯对蚜虫胁迫下菊花叶片的保护酶、防御酶活性、渗透性物质、次生代谢物和茉莉酸途径关键酶基因表达的影响,探究菊花抗蚜性与茉莉酸信号途径的关系。结果表明:0.01、0.05、0.1、0.5、1 mmol·L-1浓度的茉莉酸甲酯均显著提高了杭白菊叶片的保护酶、防御酶活性及次生代谢物含量,降低了丙二醛和可溶性糖含量,外源茉莉酸甲酯处理诱导杭白菊CmAOS和CmCOI1的表达,并使内源茉莉酸含量显著增加,杭白菊的抗蚜性增强。We examined the effects of methyl jasmonate on aphid resistance of Chrysanthemum‘Hangbai'.Leaves of chrysanthemum cuttings were sprayed by methyl jasmonic with different concentrations(0.01,0.05,0.1,0.5 and 1 mmol·L-1)and then inoculated with the aphids Macrosiphoniella sanborni.The following parameters are measured,including protective enzymes activities,defense enzyme activities,osmotic substances,secondary metabolites,and key genes of the jasmonic signaling pathway.The relationship between aphid resistance and the jasmonic signaling pathway in chrysanthemum was probed.The results showed that,under the treatments of all the five concentrations of methyl jasmonic,the activities of leaf protective enzymes and defense enzymes as well as the contents of secondary metabolite were increased in Chrysanthemum,whereas the contents of malondialdehyde and soluble sugar were reduced.Moreover,exogenous methyl jasmonate treatment induced the expression of CmAOS and CmCOI1,enhanced the content of endogenous jasmonic acid and the aphid resistance of chrysanthemum.

关 键 词：杭白菊 茉莉酸甲酯 抗蚜性 茉莉酸信号途径 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]