红松5个家系未成熟种子的胚性愈伤组织诱导研究  

Embryogenic Callus Induction of Korean Pine Immature Seeds From Five Families

作　　者：陈士刚[1] 秦彩云[1] 王聪慧 才巨锋 陶晶[1] CHEN Shigang;QIN Caiyun;WANG Conghui;CAI Jufeng;TAO Jing(Jilin Provincial Academy of Forestry Sciences,Institute of Biotechnology,Changchun 130033,China;Jilin Forest Seedling Management Station,Changchun 130022,China)

机构地区：[1]吉林省林业科学研究院林业生物技术研究所,长春130033 [2]吉林省林木种苗管理站,长春130022

出　　处：《森林工程》2021年第1期13-17,共5页Forest Engineering

基　　金：吉林省林业重大科技攻关计划项目(2015-002)。

摘　　要：红松是重要的用材树种和坚果经济林树种。以组织培养的方法,选用三岔子红松种子园5个家系的未成熟种子作为外植体进行胚性愈伤组织诱导研究。结果表明,不同家系未成熟种子在添加萘乙酸(NAA)2 mg/L+6-苄氨基嘌呤(6-BA)1 mg/L的培养基上胚性愈伤组织诱导率不同,最佳诱导率为5.68%。红松愈伤组织诱导的最适蔗糖浓度为30 g/L。选取长势良好的胚性愈伤组织进行超低温保存,在恢复培养10 d后可观察到不同细胞系均有明显新生愈伤组织,15 d后愈伤组织大量增殖。本研究为三岔子红松种子园红松优良种质资源的规模化繁殖和保存奠定了基础。Korean pine is an important timber tree species and nut economic forest tree species.The method of tissue culture was used,the immature seeds of five families from Sanchazi Korean pine seed orchard were selected as explants by tissue culture to study the embryogenic callus induction and cryopreservation.The results showed that immature seeds of different families had different induction rates of embryogenic callus on the medium supplemented with naphthaleneacetic acid(NAA)2 mg/L+6-benzylaminopurine(6-BA)1 mg/L,and the best induction percentage was 5.68%.The optimal sucrose concentration for the callus induction of Korean pine was 30 g/L.Embryogenic callus with good growth was selected for cryopreservation.After 10 days of recovery culture,it was observed that there were obvious new callus in different cell lines.After 15 days,a large number of callus proliferated.This study laid a foundation for large-scale propagation and conservation of fine germplasm resources of Pinus koraiensis in Sanchazi seed orchard.

关 键 词：红松 红松种子园 胚性愈伤组织 超低温保存 激素组合 

分 类 号：S791[农业科学—林木遗传育种]