玉米穗轴对穗腐病抗性鉴定体系与优异抗源的研究  

Study on Cob Inoculation System and Elite Resistant Inbred Lines Screened to Maize Ear Rot

作　　者：王昭 穆聪 李云梦 高景阳 宋云霞 董朝沛 马培培 赫可伟 许静 董华芳[1] 孙小东[1] 韩亚楠 周子健 陈甲法 吴建宇[1] WANG Zhao;MU Cong;LI Yun-meng;GAO Jing-yang;SONG Yun-xia;DONG Chao-pei;MA Pei-pei;HE Ke-wei;XU Jing;DONG Hua-fang;SUN Xiao-dong;HAN Ya-nan;ZHOU Zi-jian;CHEN Jia-fa;WU Jian-yu(College of Agronomy,Henan Agricultural University,Zhengzhou 450002;College of Life Science,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学农学院,郑州450002 [2]河南农业大学生命科学学院,郑州450002

出　　处：《玉米科学》2020年第6期162-167,共6页Journal of Maize Sciences

基　　金：国家自然科学基金项目(31671281,31761143009)。

摘　　要：穗轴作为拟轮枝镰孢菌侵染果穗引发玉米穗腐病的重要途径之一,能够影响病原菌的侵染和扩散。为了深入开展玉米穗轴对拟轮枝镰孢菌抗性的研究以及了解玉米穗腐病子粒与穗轴抗性的差异,进行抗性鉴定体系的优化,利用164份国内外玉米骨干自交系进行两年两点的接种鉴定筛选优异抗源,对发病边长、发病芯长、发病面积、发病相对面积等4个抗性指标进行研究。结果表明,发病边长能较大程度地区分不同自交系间的抗感差异且又在材料内具有较好的重演性。把发病边长确定为玉米穗腐病穗轴抗性鉴定指标,对参试自交系进行抗性评价,鉴定出CML27、BT-1、CML274等58份穗轴高抗自交系,筛选出BT-1、CML173、CML193等8份子粒与穗轴均抗穗腐病的优异抗源。As an important pathway,cob can affect the infection and spread of Fusarium verticillioide in maize ear.In order to study the cob resistance to Fusarium verticillioide and to understand the difference between kernel resistance and cob resistance in maize,the identification system of cob resistance was optimized.In addition,164 inbred lines were surveyed to establish the cob resistance identification system and to select excellent resistance inbred lines in two years and two locations.By analyzed of the four indexes(surrounding infected length,internal infected length,infected area and relative infected area),the surrounding infected length was identified as optimal,which has better repeatability in same inbred line and can easier distinguish the resistance between different inbred lines.Fifty-eight inbred lines with high resistance of cob were identified,such as CML27,BT-1,CML274,etc.At the same time,elite inbred lines BT-1,CML173,CML193 and other eight inbred lines with high resistance to ear rot in both kernel and cob were screened out.These results laid a foundation for disease resistance breeding and resistance inheritance research.

关 键 词：玉米 穗腐病 穗轴抗性 鉴定指标 优异抗源 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]