男男同性恋人群治疗前HIV-1流行毒株及其变异研究  

HIV-1 epidemic strains and genetic variation among therapy-naive men who have sex with men

作　　者：路新利[1] 张玉琪[1] 邱延超[2] 李保军[1] 李岩[1] 王莹莹[1] 刘萌 安宁 LU Xin-li;ZHANG Yu-qi;QIU Yan-chao;LI Bao-jun;LI Yan;WANG Ying-ying;LIU Meng;AN Ning(Department of AIDS Research,Hebei Key Laboratory of Pathogen and Epidemiology of Infectious Disease,Hebei Provincial Center for Disease Control and Prevention,Shijiazhuang 050021,China;Department of AIDS Research,Shijiazhuang Center for Disease Control and Prevention,Shijiazhuang 050011,China)

机构地区：[1]河北省疾病预防控制中心艾滋病所,河北省传染病病原学鉴定分析与流行病学重点实验室,石家庄050021 [2]石家庄市疾病预防控制中心艾滋病所,石家庄050011

出　　处：《中华疾病控制杂志》2020年第12期1465-1469,共5页Chinese Journal of Disease Control & Prevention

基　　金：河北省自然科学基金(H2016303006)。

摘　　要：目的了解掌握男男同性恋者(men who have sex with men, MSM)人群治疗前人类免疫缺陷病毒Ⅰ型(human immunodeficiency virus type one, HIV-1)流行毒株与基因变异特点。方法使用In-house方法进行耐药基因型检测,通过系统进化分析和HIV-1耐药数据库比对确定基因型和基因变异情况。结果石家庄市MSM人群中共有5种HIV-1基因型毒株在流行,CRF01AE是最主要流行毒株,占到56.8%(25/44),其次是CRF07BC 29.5%(13/44),URFs 9.1%(4/44),B亚型2.3%(1/44)和CRF5501B 2.3%(1/44)。URFs主要包括CRF01AE/B和CRF01AE/C两种重组模式。χ2检验显示不同基因型毒株在婚姻状况、年龄和首次CD4+ T淋巴细胞中的分布差异均有统计学意义(均有P<0.05)。发现1例MSM在HIV-1 NNRT编码区225位点处发生了耐药基因突变(P225H),原发耐药率为2.3%(1/44),可导致依非韦伦(EFV)和奈韦拉平(NVP)中度耐药。结论加强MSM人群中HIV-1流行和耐药监测研究对控制本市HIV疫情发展具有重要意义。Objective To explore HIV-1 genotypes and genetic variation among therapy-naive men who have sex with men(MSM) in Shijiazhuang. Methods HIV-1 genotypic resistance assay was carried out using In-house method. HIV-1 genotypes and drug resistance were analyzed by phylogenetic analysis and submitting the sequences to the Stanford University Network HIV-1 database. Results Five HIV-1 subtypes were found among MSM in Shijiazhuang. Of them, CRF01AE was the most frequent, accounting for 56.8%(25/44), followed by CRF07BC 29.5%(13/44), URFs 9.1%(4/44), B 2.3%(1/44) and CRF5501 B 2.3%(1/44). URFs mainly included CRF01AE/B and CRF01AE/C. χ2-test verified that HIV-1 genotype distribution showed significant difference(P<0.05) in marital status, age and first CD4 groups, respectively. The rate of HIV-1 primary drug resistance was 2.3%(1/44). P225 H mutation was found in NNRT gene coding region, and one MSM with P225 H occurred intermediate resistance to EFV and NVP. Conclusion It is critical for us to take measures to monitor HIV-1 epidemic and drug resistance.

关 键 词：人类免疫缺陷病毒Ⅰ型 基因型 男男同性恋者 变异 

分 类 号：R373[医药卫生—病原生物学]