碳量子点分子印迹复合材料的制备及荧光法检测甲硝唑  

Synthesis of carbon quantum dot molecularly imprinted composites and detection of metronidazole by fluorescence method

作　　者：程序森 高祥 乔子豪 于杰[1] 付瑶[1] 陈立钢[1] CHENG Xusen;GAO Xiang;QIAO Zihao;YU Jie;FU Yao;CHEN Ligang(College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin,150040)

机构地区：[1]东北林业大学化学化工与资源利用学院,哈尔滨150040

出　　处：《分析试验室》2020年第12期1416-1420,共5页Chinese Journal of Analysis Laboratory

基　　金：黑龙江省自然科学基金面上项目(JJ2018ZR0081);黑龙江省大学生创新创业训练项目(201910225347)资助。

摘　　要：采用水热合成法,以生物质废弃物玉米棒为碳源,合成了一种碳量子点。将碳量子点作为荧光组分,通过分子印迹技术,采用溶胶凝胶法一步合成了碳量子点分子印迹复合材料(CQDs@MIPs)。通过傅里叶红外变换光谱(FT-IR)、透射电子显微镜(TEM)、X射线光电子能谱(XPS)等对该材料进行表征。考察了反应时间、反应温度和反应物的质量比对碳量子点荧光强度的影响,以及激发波长、结合时间、溶剂和pH等条件对CQDs@M IPs荧光性能的影响。应用CQDs@M IPs,用荧光猝灭法对蜂蜜中的甲硝唑进行检测,在最优条件下,检测甲硝唑线性范围为25~1000 nmol/L,检出限为7.2 nmol/L,样品的加标回收率为92.3%~95.1%,相对标准偏差为2.5%~4.6%。A carbon quantum dot was synthesized by using a biomass waste corn cob as a carbon source through a hydrothermal synthesis method.Using carbon quantum dots as fluorescent components,carbon quantum dot molecularly imprinted composites(CQDs@MIPs)were synthesized by one-step sol-gel method through molecular imprinting technology.The material was characterized by Fourier transform infrared spectroscopy(FT-IR),transmission electron microscope(TEM),and X-ray photoelectron spectroscopy(XPS).The effects of reaction time,reaction temperature,and mass ratio of reactants on the fluorescence intensity of carbon quantum dots were studied.The effects of a series of conditions such as excitation wavelength,binding time,solvent and pH on the fluorescence performance of CQDs@MIPs were also investigated.The CQDs@MIPs were used to detect metronidazole in honey by fluorescence quenching.Under the optimal conditions,the linear range of metronidazole was 25-1000 nmol/L with the detection limit of 7.2 nmol/L.The spiked recoveries were92.3%-95.1%,with the relative standard deviations of 2.5%-4.6%.

关 键 词：分子印迹技术 碳量子点 荧光猝灭 甲硝唑 

分 类 号：O657.3[理学—分析化学]