CuO负载石墨烯复合材料类过氧化氢酶催化性质的研究  

Study on catalase-like catalytic properties of CuO decorated graphene nanocomposites

作　　者：曾艳 黄坤桃 吕早生 王玉华[2] 梁峰 ZENG Yan;HUANG Kuntao;LV Zaosheng;WANG Yuhua;LIANG Feng(School of Chemistry and Chemical Engineering,Wuhan University of Science and'Technology,Wuhan 430081;College of Science,Wuhan University of Science and Technology,Wuhan 430081)

机构地区：[1]武汉科技大学化学与化工学院,武汉430081 [2]武汉科技大学理学院,武汉430081

出　　处：《分析试验室》2020年第12期1439-1442,共4页Chinese Journal of Analysis Laboratory

基　　金：湖北省高等学校优秀中青年科技创新团队计划项目(T201702)资助。

摘　　要：采用原位生长法,通过控制石墨烯和CuO的质量比率,一步合成了3种不同形貌的CuO负载石墨烯纳米复合材料,并通过扫描电镜(SEM)和透射电镜(TEM)对材料进行了表征。结果表明:该材料具有类似过氧化氢酶的催化性能,催化反应与pH,反应温度以及H2O2的浓度有关,采用双倒数图解法测定了米氏常数(Km)和最大反应速率(Vmax),与辣根酶相比具有较好的催化效果。Three kinds of CuO decorated graphene nanocomposites with different morphologies were synthesized by in-situ growth method by controlling the mass ratio of graphene and CuO,and the prepared materials were characterized by scanning electron microscopy(SEM)and transmission electron microscopy(TEM).The experimental results showed that these materials had the catalytic properties similar to catalase,and the catalytic reaction was related to pH value,reaction temperature and concentration of H2O2.The Michaelis constant(Km)and the maximum reaction rate(Vmax)were determined by double reciprocal diagram method.Compared with horseradish peroxidase,it has better catalytic activity.

关 键 词：CuO负载石墨烯 过氧化氢酶 米氏常数 最大反应速率 

分 类 号：O657.91[理学—分析化学]