合并丙型肝炎病毒感染对HIV-1感染者特异性杀伤性CD4+T细胞免疫的影响  

The impact of hepatitis C virus co-infection on immune responses of specific cytotoxic CD4+ T cells among HIV-1 infected individuals

作　　者：曹孟丽 唐漾波[2] 赵稳 陈凤 王福祥 Cao Mengli;Tang Yangbo;Zhao Wen;Chen Feng;Wang Fuxiang(Department of Infectious Diseases,Shenzhen Third People’s Hospital,Shenzhen 518000,Guangdong,China;Institute of Infectious Diseases,Guangzhou Eighth People’s Hospital,Guangzhou 510060,China;Department of Respiratory Medicine,Shenzhen University General Hospital,Shenzhen 518000,Guangdong,China)

机构地区：[1]深圳市第三人民医院感染科,广东深圳518000 [2]广州市第八人民医院传染病研究所,510060 [3]深圳大学总医院呼吸科,广东深圳518000

出　　处：《国际流行病学传染病学杂志》2020年第6期488-491,共4页International Journal of Epidemiology and Infectious Disease

基　　金：国家自然科学基金 (81971915)。

摘　　要：目的探讨合并HCV感染对HIV-1感染者特异性杀伤性CD4+T细胞免疫应答的影响。方法选取2015年9月至2016年5月在广州市第八人民医院就诊的49例HIV-1感染者,根据是否合并HCV感染,分为HIV-1单纯感染组(26例)和HIV-1合并HCV感染组(23例)。以HIV-1 P24蛋白上的氨基酸序列人工合成的12个重叠肽段组成肽段库,体外刺激已接受HAART的外周CD4+T细胞,采用酶联免疫斑点试验方法检测特异性CD4+T细胞分泌IFN-γ分泌细胞个数,根据斑点形成细胞(SFC)数计算抗原特异性CD4+T细胞应答频率。以1×106个细胞产生的SFC>50,且大于各自非特异性刺激对照孔SFC的3倍作为HIV-1抗原特异性CD4+T细胞阳性反应。结果HIV-1单纯感染组的特异性杀伤性CD4+T细胞阳性应答率为80.8%,应答频率为250(100,700)SFC/106个细胞,均高于HIV-1合并HCV感染组的52.2%和50(0,250)SFC/106个细胞,组间比较差异具有统计学意义(χ2=4.538,Z=-2.432,P均<0.05)。结论HIV-1感染者合并HCV感染可能抑制特异性杀伤性CD4+T细胞反应。Objective To investigate the effect of HIV-1 co-infection with HCV on immune responses of HIV-1 specific cytotoxic CD4+T cells.Methods There were 49 confirmed HIV-1 infected patients selected at Guangzhou Eighth People’s Hospital from September 2015 to May 2016.According to whether they were co-infected with HCV,26 and 23 patients were assigned to HIV-1 infection group and HIV-1/HCV co-infection group,respectively.The peripheral CD4+T cells of the patients who had received HAART were stimulated in vitro by a peptide pool containing 12 overlapping peptides that synthesized from the amino acid sequence of HIV-1 P24 protein.The number of IFN-γ-secreting cells secreted by specific CD4+T cells was detected by enzyme-linked immunospot assay.The response frequency of antigen-specific CD4+T cells was calculated by the number of spot-forming cells(SFC).If SFC produced by 1×106 cells was more than 50,and 3 times greater than that in respective non-specific stimulus control wells,it was defined as positive response of HIV-1 antigen-specific CD4+T cell.Results The positive response rate of specific cytotoxic CD4+T cells in HIV-1 infection group was 80.8%,and the response frequency was 250(100,700)SFC/106 cells,which were both higher than those of 52.2%and 50(0,250)SFC/106 cells in HIV-1/HCV co-infection group.The differences between the two groups were statistically significant(χ2=4.538,Z=-2.432,P both<0.05).Conclusions HCV co-infection in HIV-1 infected individuals may inhibit responses of specific cytotoxic CD4+T cells.

关 键 词：HIV感染 肝炎病毒 丙型 杀伤性CD4+T细胞 抑制 

分 类 号：R512.91[医药卫生—内科学] R512.63[医药卫生—临床医学]