血型血清学结合PCR-SSP基因分型法在新生儿ABO血型鉴定中的应用  

Application of blood group serology combined with PCR-SSP genotyping in the identification of ABO blood group in newborns

作　　者：尹明伟 陈学军[1] 徐军[1] 金小波[1] 周超[1] 蔡晟阳 YIN Mingwei;CHEN Xuejun;XU Jun;JIN Xiaobo;ZHOUChao;CAI Shengyang(Blood Transfusion Department Central Laboratory Children′s Hospital,Zhejiang University School of Medicine,National Clinical Research Center for Child Health,Hangzhou 310052,China)

机构地区：[1]浙江大学医学院附属儿童医院实验检验中心输血科国家儿童健康与疾病临床医学研究中心,浙江杭州310052

出　　处：《中国输血杂志》2020年第10期1012-1016,共5页Chinese Journal of Blood Transfusion

基　　金：浙江省血液安全研究重点实验室开放基金资助项目(2018KF02)。

摘　　要：目的建立ABO血型基因的PCR检测方法,评价常规ABO血清学正定法用于新生儿ABO定型的可靠性,探讨血型血清学结合PCR-SSP基因分型法在新生儿ABO血型鉴定中的价值。方法采用血清学方法鉴定110例新生儿血型,结合PCR-SSP基因分型方法做检测,并对其中4例PCR-SSP方法未明确血型的通过测序确定ABO基因型。结果110例新生儿ABO血型标本,血清学检测方法中,2例抗原强度较弱,无法诊断血型,通过PCR-SSP方法诊断为AB型;PCR-SSP检测方法中,106例确定基因型,PCR-SSP方法未明确血型的4例标本通过测序确定基因型,所有基因分型结果与血清学检测结果一致率为100%;新生儿ABO血型正反定型不符率为63%,A、B、O型正反定不符率比较,P>0.05。结论ABO血型基因分型结合血清学分型可用于新生儿ABO血型鉴定,一定程度上可弥补血清学方法的不足。Objective To establish the molecular genetic blood group typing based on PCR-SSP,so as to assess the reliability of direct antigendetermiantson RBCs for the newborns,and discuss the value of the serological typing combined with PCR-SSP for ABO typing in neonates.Methods The blood groups of 110 neonates were identified by serological typing combined with PCR-SSP.The 4 unspecified cases by PCR-SSP were further validated by gene sequencing.Results Among 110 neonatal ABO blood group samples,108 were identified by serological typing,and 2 were successfully confirmed as group AB by PCR-SSP due to the weakened antigen expression.106 cases were genotyped by PCR-SSP,and 4 equivocal cases were further confirmed by gene sequencing.The geno typing results were in full concordance with serological phenotypes.The incidence of discrepancies between direct and indirectantigen determinantsinneonates was 63%,showing no significant differences in the incidences among group A,B and O(P>0.05).Conclusion ABO blood group genotyping combined with serological test can be used for neonatal ABO blood group identification,and can make up the deficiency of serological method to some extent.

关 键 词：ABO基因型分型 血清学分型 新生儿 血型鉴定 

分 类 号：R457.11[医药卫生—治疗学]