岳阳汉族人群10个红细胞血型系统基因频率及多态性研究  

Study on the gene frequency and polymorphism of 10 RBC blood group systems in Han population in Yueyang,China

作　　者：孙昂 苏湘晖 陈宏伟 易玲 谭涛 向雅香 孔维臣 SUN Ang;SU Xianghui;CHEN Hongwei;YI Ling;TAN Tao;XIANG Yaxiang;KONG Weichen(Yueyang Blood Station,Yueyang 414000,China;Changchun Brother Biotechnology Co.Ltd.)

机构地区：[1]岳阳市中心血站,湖南岳阳414000 [2]长春博德生物技术有限责任公司

出　　处：《中国输血杂志》2020年第10期1033-1037,共5页Chinese Journal of Blood Transfusion

基　　金：湖南省自然科学基金面上项目(2019JJ40292)。

摘　　要：目的了解岳阳汉族人群Rh(C/c/E/e),Kidd(Jka/Jkb),MNS(M/N/S/s/Mur),Duffy(Fya/Fyb),Diego(Dia/Dib),Kell(K/k/Kpa/Kpb),Dombrock(Doa/Dob),Scianna(Sc1/Sc2),Colton(Coa/Cob),Lutheran(Lua/Lub)10个红细胞血型系统的基因频率和多态性特点,为建立本地区稀有血型库提供数据。方法采用PCR-SSP方法对303名岳阳汉族RhD(+)固定献血者进行10个血型系统基因分型,并进行统计分析。结果岳阳汉族人群RhCE血型系统基因频率为:C=0.6865,c=0.3135,E=0.2492,e=0.7508;MNS血型基因频率为:M=0.4983,N=0.5017,S=0.0478,s=0.9521,Mur=0.0363;Duffy血型的基因频率为Fya=0.9472,Fyb=0.0528;Dombrock血型基因频率为:Doa=0.0842,Dob=0.9158;Diego血型基因频率为:Dia=0.0247,Dib=0.9752;Kidd血型基因频率为:Jka=0.4802,Jkb=0.5198。303名献血者Lutheran血型、Kell血型、Scianna血型和Colton血型均为纯合子,基因型分别为Lub/Lub、k/k/Kpa/Kpb、Scl/Scl和Coa/Coa。所检测的基因型均符合Hardy-Weinberg平衡法则。结论岳阳汉族人群RhCE、MNS、Duffy、Diego、Kidd和Dombrock血型系统基因呈多态性分布,Lutheran和Colton、Kell、Scianna血型系统基因呈单态性分布。Objective To investigate the gene frequency and polymorphism of 10 RBC blood group systems,including Rh(C/c/E/e),Kidd(Jk^a/Jk^b),MNS(M/N/S/s/Mur),Duffy(Fy^a/Fy^b),Diego(Di^a/Di^b),Kell(K/k/Kp^a/Kp^b),Dombrock(Do^a/Do^b),Scianna(Sc1/Sc2),Colton(Co^a/Co^b)and Lutheran(Lu^a/Lu^b)in Han population inYueyan,aimed to provide data for the establishment of rare blood group registry in this region.Methods The genotypes of ten blood groups of RhD(+)samples from 303 regular blood donors in Yueyang were analyzed by PCR-SSP and then analyzed statistically.Results The gene frequency of ten blood group systems were as follows:RHCE:C=0.6865,c=0.3135,E=0.2492,e=0.7508;MNS:M=0.4983,N=0.5017,S=0.0478,s=0.9521,Mur=0.0363;Duffy:Fy^a=0.9472,Fy^b=0.0528;Dombrock:Do^a=0.0842,Do^bb=0.9158;Diego:Di^a=0.0274,Di^b=0.9752;Kidd:Jk^a=0.4802,Jk^b=0.5198.Lutheran,Kell Scianna and Colton blood groups in the 303 samples were all homozygous,and with respective genetypes as Lu^b/Lu^b,k/k/Kp^a/Kp^b,Scl/Scl and Co^a/Co^a.The genotypes detected in the ten systems complied with Hardy-Weinberg equilibrium.Conclusion The RhCE,MNS,Duffy,Diego,Kidd and Dombrock blood group systems in Han population in Yueyang showed polymorphic distribution,while the Lutheran,Colton,Kell and Scianna showed monomorphic distribution.

关 键 词：红细胞血型 基因频率 基因多态性 Hardy-Weinberg平衡 岳阳汉族固定献血者 

分 类 号：R457.11[医药卫生—治疗学]