象皮生肌膏对慢性创面模型大鼠血清β-EP、PGE2和5-HT表达的影响  

Effect of Xiangpi Shengji Ointment(象皮生肌膏)on the Expression ofβ-EP,PGE2 and 5-HT in Serum of Chronic Wound Model Rats

作　　者：杜攀攀 李木清[2] 毛滔[2] 刘梨[3] 程学荣 杨振武 姜雄 DU Pan-pan;LI Mu-qing;MAO Tao;LIU Li;CHENG Xue-rong;YANG Zhen-wu;JIANG Xiong(Hunan University of Traditional Chinese Medicine,Changsha Hunan 410208,China;The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha Hunan 410005,China;The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha Hunan 410007,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学第二附属医院,湖南长沙410005 [3]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中医药导报》2020年第16期31-34,44,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省科技厅临床医疗技术创新引导项目(2018SK51101)。

摘　　要：目的:研究象皮生肌膏对慢性创面模型大鼠血清β-EP、PGE2和5-HT表达的影响,探讨象皮生肌膏对慢性创面模型大鼠的镇痛作用机制。方法:将72只SD雄性大鼠随机分为正常组、模型组、对照组及实验组,每组18只。正常组大鼠仅备皮处理,其余大鼠备皮后在背部制备直径20 mm深至皮下深筋膜层的圆形创面,再经腹腔注射醋酸氢化可的松制备慢性创面模型;正常组及模型组大鼠给予生理盐水纱布外敷,对照组大鼠予重组牛碱性成纤维细胞生长因子凝胶外涂,实验组大鼠予象皮生肌膏外涂。每组分别于第3、7、14天随机选择6只大鼠处死经腹主动脉采血,采用ELISA法检测血清β-EP、PGE2和5-HT表达水平,并取创面组织HE染色观察组织形态。结果:对照组及实验组大鼠在第7天和第14天生长情况及病理形态学优于模型组,第3天3组无明显差异。正常组、对照组及实验组大鼠第3、7、14天的血清β-EP表达水平均高于模型组(P<0.05),血清PGE2及5-HT表达水平均低于模型组(P<0.05);对照组和实验组大鼠在第3、7、14天血清β-EP、PGE2及5-HT的表达水平比较,差异均无统计学意义(P<0.05)。时间与组别的交互作用对血清β-EP、PGE2及5-HT表达水平的影响差异无统计学意义(P>0.05)。大鼠不同时间血清β-EP、PGE2和5-HT表达水平比较,差异均无统计学意义(P>0.05)。结论:象皮生肌膏可升高慢性创面模型大鼠血清中β-EP表达水平,降低血清中PGE2及5-HT表达水平。调控β-EP、PGE2及5-HT的表达水平可能是象皮生肌膏镇痛的作用机制之一。Objective: To study the effect of Xiangpi Shengji Ointment on the expression of serum β-EP,PGE2 and 5-HT in chronic wound model rats, and to explore its analgesic mechanism. Methods: A total of 72 SD male rats were randomly divided into normal group, model group, control group and experimental group, with18 rats in each group. The rats in the normal group were only treated with skin preparation. After skin preparation, a round wound with a diameter of 20 mm to the deep subcutaneous fascia layer was prepared on the back of the other rats, and then hydrocortisone acetate was injected intraperitoneally to establish the chronic wound model. Rats in the normal group and model group were externally applied with saline gauze. Rats in the control group were externally coated with recombinant bovine basic fibroblast growth factor gel, and rats in the experimental group were externally coated with Xiangpi Shengji Ointment. Six rats in each group were randomly se-lected on the 3 rd, 7 thand 14 thdays respectively. Blood samples were collected through abdominal aorta. The expression levels of serum β-EP, PGE2 and 5-HT were detected by ELISA, and the tissue morphology was observed by HE staining. Results: The growth and pathological morphology of the rats in the control group and the experimental group were better than those in the model group on the 7 thand 14 thday. There was no significant difference among the three groups on the 3 rdday. The expression levels of serum β-EP in the normal group,control group and experimental group on the 3 rd, 7 thand 14 thday were higher than that of the model group(P<0.05), and the expression levels of serum PGE2 and 5-HT were lower than the model group(P<0.05). The interaction of time and group had no significant difference in the effect of serum β-EP, PGE2 and 5-HT expression levels(P>0.05). There was no significant difference in serum β-EP, PGE2 and 5-HT expression levels at different times(P>0.05). Conclusion: Xiangpi Shengji Ointment can increase the expression of β-EP in the s

关 键 词：慢性创面 象皮生肌膏 Β-内啡肽 前列腺素E2 5-羟色胺 大鼠 

分 类 号：R285.5[医药卫生—中药学]