KDM3A沉默对人乳腺癌细胞系MDA-MB-231凋亡和侵袭能力的影响  

Effects of KDM3A silencing on the apoptosis and invasion of human breast cancer cell line MDA-MB-231

作　　者：张天瑞 高文怡 姚娟 ZHANG Tian-rui;GAO Wen-yi;YAO Juan(Xinjiang Medical University, Urumqi 830000;Imaging Center, Department of Radiology, the First Affiliated Hospital,Xinjiang Medical University, Urumqi 830000, China)

机构地区：[1]新疆医科大学,新疆乌鲁木齐830000 [2]新疆医科大学第一附属医院影像中心,新疆乌鲁木齐830000

出　　处：《基础医学与临床》2021年第1期33-37,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81960339,81660305)。

摘　　要：目的探讨siRNA介导的KDM3A基因沉默对乳腺癌细胞系MDA-MB-231增殖、凋亡及侵袭的影响。方法构建KDM3A基因特异性siRNA慢病毒载体。感染MDA-MB-231细胞,实时荧光定量PCR检测乳腺癌细胞转染siRNA-KDM3A的效率;CCK-8法检测细胞增殖;Western blot检测细胞凋亡;Transwell小室法检测细胞侵袭。结果在MDA-MB-231细胞中,相对于NC组,KDM3A-sh1、KDM3A-sh2、KDM3A-sh3组中KDM3A基因的mRNA表达均下调;KDM3A-sh2组基因敲低效率最高;mRNA相对表达量降低了80.1%(P<0.01);沉默KDM3A基因后,MDA-MB-231细胞增殖能力减弱,凋亡率升高(P<0.05),侵袭能力下降(P<0.01)。结论siRNA介导的KDM3A沉默能抑制乳腺癌细胞的增殖、侵袭,并促进细胞凋亡。Objective To investigate the effect of siRNA mediated KDM3A gene silencing on cell proliferation and apoptosis of breast cancer cell line MDA-MB-231.Methods Constructed a KDM3A gene-specific siRNA lentiviral vector to infect breast cancer MDA-MB-231 cells,and detected the efficiency of breast cancer cells transfected with siRNA-KDM3A by the real-time fluorescent quantitative PCR.CCK-8 method was used to detect cell proliferation,Western blot was used to test cell apoptosis and Transwell method was used to detect cell invasion.Results Compared with the control group,the expression of KDM3A mRNA was down-regulated in KDM3A-sh1,KDM3A-sh2 and KDM3A-sh3 cells,and the most efficient interference RNA was KDM3A-sh2,with the expression of mRNA decreased by 80.1%(P<0.01).After silencing KDM3A,the proliferation of MDA-MB-231 cells decreased,the apoptosis rate increased(P<0.05),and the invasion ability decreased(P<0.01).Conclusions siRNA-mediated KDM3A gene silencing inhibits proliferation and invasion of breast cancer cells and promotes their apoptosis.

关 键 词：KDM3A 基因沉默 乳腺癌 增殖 凋亡 

分 类 号：R34[医药卫生—基础医学]