黄芪主要成分对TGF-β1诱导大鼠睾丸Leydig细胞系合成雌二醇的影响  

Effects of main compositions of Astragalus on estradiol synthesizing activing in TGF-β1-induced rat testicular Leydig cell line

作　　者：李彦荣 刘光炜 赵晶 李亚军 LI Yan-rong;LIU Guang-wei;ZHAO Jing;LI Ya-jun(Gansu University of Traditional Chinese Medicine(Dingxi Campus), Dingxi 743000;Department of Clinical Laboratory,Dingxi People's Hospital, Dingxi 743000, China)

机构地区：[1]甘肃中医药大学(定西校区),甘肃定西743000 [2]定西市人民医院检验科,甘肃定西743000

出　　处：《基础医学与临床》2021年第1期68-71,共4页Basic and Clinical Medicine

基　　金：2018年度甘肃省高等学校科研项目(2018A-180);甘肃中医药大学定西校区项目(2017XJZD09)。

摘　　要：目的探讨黄芪主要成分对TGF-β1诱导下大鼠睾丸Leydig细胞及该细胞合成雌二醇(E2)、P450芳香化酶(P450arom)的影响。方法将体外培养的Leydig细胞分为对照组、模型组(5 ng/mL TGF-β1造模)和中药干预3组,在造模后分别用终浓度为10和50 mg/L黄芪总黄酮、终浓度为10和50 mg/L黄芪总多糖、终浓度20和100 mg/L的黄芪总皂苷干预。48 h后进行细胞爬片,用ELISA检测细胞中E2、P450arom蛋白含量;Imagej-win64软件计数细胞;用SPSS 20.0、GraphPad Prism 6.0对数据进行统计分析。结果模型组Leydig细胞和对照组相比,形态异常,数量减少(P<0.01);明显抑制Leydig细胞合成E2和P450arom(P<0.05)。黄芪总黄酮、黄芪总皂苷和黄芪总多糖均能够改善TGF-β1诱导下Leydig细胞病变,细胞数量明显增多(P<0.01),三者均能缓解TGF-β1抑制细胞合成E2的作用,20 mg/L黄芪总皂苷的效果最显著。结论黄芪总皂苷是黄芪中减轻TGF-β1抑制睾丸Leydig细胞合成E2作用效果最佳的成分。Objective To investigate the effects of the main compositions of Astragalus on testicular Leydig cells and their synthesis of estradiol(E2),P450arom under TGF-β1 induction.Methods Leydig cells were divided into three groups:control group,model group and Chinese herbal medicine intervention group.Results Compared with the control group,Leydig cells in the model group showed abnormal morphology and less cells(P<0.01).TGF-β1 significantly inhibited the synthesis of E2 and P450arom by Leydig cells(P<0.05).Total Astragalus flavone,total Astragalus saponin and total astragalus polysaccharide all resulted in pathological changes of Leydig cells induced by TGF-β1.Compared with the model group,the number of cells increased significantly(P<0.01)and all of them alleviated the inhibitory effect of TGF-β1 on the synthesis of E2 in Leydig cells induced by TGF-β1,and the effect of concentration of 20 mg/L total saponin of Astragalus was the most significant.Conclusions Total Astragalus saponin is the most effective component in Astragalus to exert TGF-β1 inhibitory effect on E2 synthesis by Leydig cells.

关 键 词：黄芪 睾丸Leydig细胞 雌二醇(E2) 

分 类 号：R285.5[医药卫生—中药学]