α-硫辛酸对自身免疫性甲状腺炎小鼠炎性反应的抑制作用  

Inhibition of inflammatory response byα-lipoic acid in autoimmune thyroiditis mice

作　　者：玛依努·玉苏甫[1] 衣巴地古丽·库吐鲁克 哈尼克孜·阿不都艾尼[1] Mayinu YUSUFU;Yibadiguli KUTURUKE;Hanikezi ABUDUAINI(Department of Endocrinology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830000, China)

机构地区：[1]新疆维吾尔自治区人民医院内分泌科,新疆乌鲁木齐830000

出　　处：《基础医学与临床》2021年第1期72-76,共5页Basic and Clinical Medicine

基　　金：新疆维吾尔自治区自然科学基金(2017D01C106)。

摘　　要：目的探讨α-硫辛酸抑制自身免疫性甲状腺炎(AIT)小鼠炎性反应及对淋巴细胞亚群的影响。方法将80只NOD.H-2h 4雌鼠随机分为对照组,给予无菌双蒸水灌胃;AIT组给予50 mg/L碘化钠无菌双蒸水灌胃;低及高剂量α-硫辛酸干预组每天腹腔注射0.1和0.4 g/Lα-硫辛酸。每组20只,均持续干预8周。干预8周后,用HE染色观察淋巴细胞浸润;采用流式细胞仪检测Th17、Treg、CD4^+、CD8^+T细胞及CD4^+/CD8^+水平;采用Western blot测定p38MAPK、NF-κB蛋白水平。结果AIT组小鼠甲状腺炎性反应发生率高达85%。AIT+低/高剂量α-硫辛酸组小鼠甲状腺炎发生率、甲状腺炎性反应程度评分以及Th17细胞比例显著低于AIT组(P<0.05);Treg、CD4^+、CD8^+以及CD4^+/CD8^+细胞比例显著高于AIT组(P<0.05)。AIT+低/高剂量α-硫辛酸组的蛋白相对表达水平较AIT组明显降低(P<0.05)。结论α-硫辛酸可以降低AIT小鼠炎性反应因子的表达,改善淋巴细胞亚群的比例,从而抑制炎性反应和改善AIT症状。Objective To investigate the inhibitory effect ofα-lipoic acid on inflammation in autoimmune thyroiditis(AIT)mice and its effect on lymphocyte subsets.Methods Eighty NOD.H-2h 4 female rats were randomly divided into a control group and the AIT group which was given 50 mg/L sodium iodide orally;Low-and high-doseα-lipoic acid intervention group was injected intraperitoneally with 0.1 and 0.4 g/Lα-lipoic acid daily.Twenty animals in each group were continued to be intervened for 8 weeks.After 8 weeks of intervention,HE staining was used to observe lymphocyte infiltration;flow cytometry was used to detect Th17,Treg,CD4^+,CD8^+T cells and CD4^+/CD8^+levels;and Western blot method was used to determine p38MAPK and NF-κB protein level.Results The incidence of thyroid inflammation in AIT group was as high as 85%.The incidence of thyroiditis,the degree of thyroid inflammation,and the proportion of Th17 cells in the AIT+low/high-dose alpha-lipoic acid group were significantly lower than those in the AIT group(P<0.05),and the proportion of Treg,CD4^+,CD8^+,and CD4^+/CD8^+cells were significant higher than that in the AIT group(P<0.05).The AIT+low/high-doseα-lipoic acid group had significantly lower relative protein expression than those in the AIT group(P<0.05).Conclusionsα-lipoic acid can reduce the expression of inflammatory cytokines in autoimmune thyroid mice,improve the proportion of lymphocyte subsets,thereby suppressing the inflammatory response and improving the symptoms of AIT.

关 键 词：Α-硫辛酸 自身免疫性甲状腺炎 p38促分裂原活化蛋白激酶(p38MAPK) 核因子-κB通路 T淋巴细胞亚群 

分 类 号：R584.1[医药卫生—内分泌]