4,4'-二甲氧基查耳酮改善高脂饲养小鼠的胰岛素敏感性  

4,4'-Dimethoxy chalcone improves insulin sensitivity of mice in high-fat diet

作　　者：张伟虹 赵微 李春美 解相宏 姚红[1] 刘晓军 ZHANG Wei-hong;ZHAO Wei;LI Chun-mei;XIE Xiang-hong;YAO Hong;LIU Xiao-jun(Department of Microbiology and Immunology, Basic Medicine College, Shanxi Medical University, Taiyuan 030001;National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences CAMS,School of Basic Medicine PUMC, Beijing 100005, China)

机构地区：[1]山西医科大学基础医学院微生物学与免疫学教研室,山西太原030001 [2]中国医学科学院基础医学研究所,北京协和医学院基础学院医学分子生物学国家重点实验室,北京100005

出　　处：《基础医学与临床》2021年第1期77-81,共5页Basic and Clinical Medicine

基　　金：中国医学科学院医学与健康科技创新工程(CIFMS2017-I2M-1-008)。

摘　　要：目的观察黄酮类药物4,4'-二甲氧基查耳酮(DMC)在高脂饲养的小鼠中胰岛素敏感性的变化,并探讨其作用机制。方法将高脂喂养的野生型C57/BL 6小鼠分为对照组和实验组每隔1 d按3μg/kg腹腔分别注射药物DMC和溶剂DMSO。每周检测记录两组小鼠进食和体质量,在第6周检测小鼠糖耐量(GTT)、胰岛素耐量(ITT)和丙酮酸钠耐量(PTT)实验;用免疫组化和HE染色检测小鼠脂肪组织变化。结果与对照组小鼠相比,DMC实验组小鼠在给药后体质量及进食差异无统计学意义。ITT实验结果显示DMC实验组小鼠胰岛素水平得到改善(P<0.05),GTT和PTT实验结果差异无统计学意义。脂肪组织HE染色显示对照组小鼠脂肪组织内有炎性细胞渗出,F4/80免疫组化染色结果提示DMC实验组小鼠脂肪组织内巨噬细胞浸润减少。结论药物DMC可以通过减少脂肪组织内巨噬细胞浸润改善高脂饲养小鼠的胰岛素敏感性。Objective To observe the changes of insulin sensitivity of flavonoids 4,4'-dimethoxy chalcone(DMC)in mice fed on high-fat diet(HFD)and to investigate its effect.Methods Wild-type C57/BL 6 mice fed on HFD were divided into control group and experimental group.DMC and solvent DMSO were injected intraperitoneally at 3μg/kg every other day.Food intake and body weight of the two groups were recorded weekly,glucose tolerance test(GTT),insulin tolerance test(ITT)and sodium pyruvate tolerance test(PTT)were measured at week 6.The adipose tissues were examined by immunohistochemistry and HE staining.Results Compared with the control mice,the mice treated with DMC drug was no different in body weight and food intake.ITT indicated that DMC administration increased insulin sensitivity(P<0.05),but GTT and PTT showed no significant difference.HE staining microscopy showed that adipose tissue contained less mononuclear cells in mice treated with DMC than control mice.There was weak staining for F4/80 in the adipose tissue of mice treated with DMC,defined as F4/80-positive cells.Conclusions DMC can improve the insulin sensitivity of mice in high-fat diet by decreasing the infiltration of macrophages in adipose tissue.

关 键 词：4 4'-二甲氧基查耳酮(DMC) 脂肪组织炎性反应 胰岛素敏感性 

分 类 号：R587.1[医药卫生—内分泌]