超高效液相色谱串联质谱法测定水体中17种抗生素  

Determination of 17 Antibiotics in Drinking Water Sources in Zhejiang Province by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：陈书鑫 王静 何士冲 刘铮铮 冯华军[1] 穆朋倩 CHEN Shuxin;WANG Jing;HE Shichong;LIU Zhengzheng;FENG huajun;MU Pengqian(Zhejiang Key Laboratory of Solid Waste Treatment and Recycling,School of Environmental Science and Engineering,Zhejiang Gongshang University,Hangzhou 310012,China;Zhejiang Ecological Environment Monitoring Center,Hangzhou 310012,China;China Asia Pacific Application Support Center,AB SCIEX,Shanghai 200050,China)

机构地区：[1]浙江工商大学环境科学与工程学院,浙江省固体废物处理处置与资源化重点实验室,浙江杭州310012 [2]浙江省生态环境监测中心,浙江杭州310012 [3]中国亚太应用支持中心,AB Sciex,上海200050

出　　处：《中国环境监测》2020年第6期119-126,共8页Environmental Monitoring in China

基　　金：浙江省环保科研计划项目(2016A011)。

摘　　要：建立了一种超高效液相色谱串联质谱法同时测定饮用水源中17种超痕量水平抗生素的分析方法。采用Oasis HLB柱对500 mL pH=5含目标物的水样进行富集,干燥15 min后,用5%氨水甲醇洗脱,氮吹后定容至1 mL。用C18柱吸附目标物,并用0.1%甲酸水和甲醇对目标物进行分离,采用正离子电喷雾电离模式在多反应监测通道中对目标物同时进行分析。该方法检出限为0.07~0.78 ng/L,超痕量水平下回收率范围为75.8%~108%。该方法灵敏度高,精密度为1.8%~15.0%,适用于同时测定饮用水源等水体中的抗生素。An analytical method was developed for the simultaneous determination of 17 ultra-trace levels of antibiotics in drinking water sources by ultra-performance liquid chromatography-tandem mass spectrometry.Oasis HLB column was used to enrich 500 mL water samples containing the target compounds with pH = 5.After drying for 15 min,elution was conducted with 5%ammonia methanol,and then blow the extracted liquid nitrogen to 1 mL.The target was adsorbed by C18 column and separated by 0.1% formic acid water and methanol.The target was analyzed simultaneously in the multi-reaction monitoring channel by using positive ion electrospray ionization mode.The detection limit was 0.07-0.78 ng/L,and the recovery at ultra-trace level was between 75.8%-108%.The method has high sensitivity,high efficiency and stable precision of 1.8%-15.0%,which can be used for simultaneous determination of antibiotics in drinking water sources,surface water and other water bodies.

关 键 词：抗生素 固相萃取 超高效液相色谱串联质谱法 饮用水源 

分 类 号：X830.2[环境科学与工程—环境工程]