灌木型柳树杂种无性系铅的积累特性及其遗传变异  

Lead accumulation characteristics and genetic variation of shrub willow hybrid clones

作　　者：黄瑞芳[1,2] 王红慧 王红玲[1,2] 施士争[1,2] Huang Ruifang;Wang Honghui;Wang Hongling;Shi Shizheng(Jiangsu Academy of forestry,Nanjing 211153,China;The Jiangsu Provincial Infrastructure for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014,China;Jiangsu Agricultural Product Quality Inspection and Testing Center,Nanjing 210036,China)

机构地区：[1]江苏省林业科学研究院,江苏南京211153 [2]江苏省农业种质资源保护与利用平台,江苏南京210014 [3]江苏省农产品质量检验测试中心,江苏南京210036

出　　处：《江苏林业科技》2020年第6期28-33,41,共7页Journal of Jiangsu Forestry Science & Technology

基　　金：江苏省农业科技自主创新资金项目“灌木柳树修复铅污染土壤的专用品种选育”[CX(19)3044];江苏省林业科技创新与推广项目“灌木柳新品种的引种繁育与示范”(LYKJ-邳州[2020]03);江苏省林业科技创新与推广项目“沿海地区高标准农田林网建设关键技术”(LYKJ[2018]03)。

摘　　要：为研究灌木柳树的铅积累特性及其遗传变异差异,以8个杂交组合的51个灌木柳树无性系为试验材料,通过水培法测定了其在铅(300μM)胁迫状况下的成活率、受害指数,分别根、茎、叶测定了其生物量、铅含量和铅总量。结果显示:不同杂交组合的存活率、受害指数和生物量平均值差异显著,P126×P585的子代受害指数为(3.70±2.27)%,显著小于其他杂交组合,P126×P63的子代的生物量为(13.85±4.95)g,显著大于其他杂交组合。杂交组合P1025×P63的成活率、受害指数的变异均最大,变异系数分别为51.65%和63.11%,生物量变异最大的为P294×P63杂交组合,变异系数达到79.97%。灌木柳树根部铅总量占整株铅总量的64.47%—91.28%,茎部铅总量占比为3.70%—16.08%,叶部铅总量占比为4.45%—20.72%,表明灌木柳树在受到铅胁迫时,铅主要积累在根部。铅含量变异最大的为P336×P63杂交组合,变异系数为50.79%,铅总量变异最大的为P61×P63杂交组合,变异系数为45.91%。差异分析表明,铅含量和铅总量在不同杂交组合间的差异均不显著,而无性系间差异显著,在今后的良种选择上可以直接从无性系中选择。In order to study the characteristics of lead accumulation and genetic variation of shrub willow,51 clones of 8 hybrid combinations were used as experimental materials.The survival rate and damage index of them under lead pollution(300μm)were measured by hydroponics method.Their biomass,lead content and total lead amount were measured in roots,stems and leaves.The results were as followed,there were some significant differences in average biomass,survival and damage index among different hybrid combinations,with(3.70±2.27)%damage index of P126×P585,significantly lower than those of the others,(13.85±4.95)g biomass of P126×P63,significantly higher.And the survival and damage index of P1025×P63 had the largest variations,with variation coefficient of 51.65%and 63.11%respectively.P294×P63 had the biggest variation of biomass with variation coefficient of 79.97%.The total amount of lead in root accounted for 64.47%—91.28%,3.70%—16.08%in the stem and 4.45%—20.72%in the leaf,indicating that the lead mainly accumulated in roots under lead stress.There occurred the biggest variation of lead content in P336×P63 and biggest variation of total lead amount in P61×P63,with variation coefficient of 50.79%and 45.91%respectively.No significant differences were found in lead content and total lead amount among different hybrid combinations,but significant differences among clones,prompting the selection of improved varieties could be directly selected at clone level.

关 键 词：灌木柳树 无性系 铅胁迫 积累特性 遗传变异 

分 类 号：Q945.78[生物学—植物学] Q948.116